Filopodia rotate and coil by actively generating twist in their actin shaft

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Standard

Filopodia rotate and coil by actively generating twist in their actin shaft. / Leijnse, Natascha; Barooji, Younes Farhangi; Arastoo, Mohammad Reza; Sonder, Stine Lauritzen; Verhagen, Bram; Wullkopf, Lena; Erler, Janine Terra; Semsey, Szabolcs; Nylandsted, Jesper; Oddershede, Lene Broeng; Doostmohammadi, Amin; Bendix, Poul Martin.

I: Nature Communications, Bind 13, Nr. 1, 1636, 28.03.2022.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Leijnse, N, Barooji, YF, Arastoo, MR, Sonder, SL, Verhagen, B, Wullkopf, L, Erler, JT, Semsey, S, Nylandsted, J, Oddershede, LB, Doostmohammadi, A & Bendix, PM 2022, 'Filopodia rotate and coil by actively generating twist in their actin shaft', Nature Communications, bind 13, nr. 1, 1636. https://doi.org/10.1038/s41467-022-28961-x

APA

Leijnse, N., Barooji, Y. F., Arastoo, M. R., Sonder, S. L., Verhagen, B., Wullkopf, L., Erler, J. T., Semsey, S., Nylandsted, J., Oddershede, L. B., Doostmohammadi, A., & Bendix, P. M. (2022). Filopodia rotate and coil by actively generating twist in their actin shaft. Nature Communications, 13(1), [1636]. https://doi.org/10.1038/s41467-022-28961-x

Vancouver

Leijnse N, Barooji YF, Arastoo MR, Sonder SL, Verhagen B, Wullkopf L o.a. Filopodia rotate and coil by actively generating twist in their actin shaft. Nature Communications. 2022 mar. 28;13(1). 1636. https://doi.org/10.1038/s41467-022-28961-x

Author

Leijnse, Natascha ; Barooji, Younes Farhangi ; Arastoo, Mohammad Reza ; Sonder, Stine Lauritzen ; Verhagen, Bram ; Wullkopf, Lena ; Erler, Janine Terra ; Semsey, Szabolcs ; Nylandsted, Jesper ; Oddershede, Lene Broeng ; Doostmohammadi, Amin ; Bendix, Poul Martin. / Filopodia rotate and coil by actively generating twist in their actin shaft. I: Nature Communications. 2022 ; Bind 13, Nr. 1.

Bibtex

@article{4a5125f8960e4046ab1164bae3aaddad,
title = "Filopodia rotate and coil by actively generating twist in their actin shaft",
abstract = "The authors show how tubular surface structures in all cell types, have the ability to twist and perform rotary sweeping motion to explore the extracellular environment. This has implications for migration, sensing and cell communication.Filopodia are actin-rich structures, present on the surface of eukaryotic cells. These structures play a pivotal role by allowing cells to explore their environment, generate mechanical forces or perform chemical signaling. Their complex dynamics includes buckling, pulling, length and shape changes. We show that filopodia additionally explore their 3D extracellular space by combining growth and shrinking with axial twisting and buckling. Importantly, the actin core inside filopodia performs a twisting or spinning motion which is observed for a range of cell types spanning from earliest development to highly differentiated tissue cells. Non-equilibrium physical modeling of actin and myosin confirm that twist is an emergent phenomenon of active filaments confined in a narrow channel which is supported by measured traction forces and helical buckles that can be ascribed to accumulation of sufficient twist. These results lead us to conclude that activity induced twisting of the actin shaft is a general mechanism underlying fundamental functions of filopodia.",
keywords = "EMBRYONIC STEM-CELLS, GROUND-STATE, DYNAMICS, CYTOSKELETON, ADHESION, FASCIN, INVADOPODIA, RETRACTION, MAINTAINS, MIGRATION",
author = "Natascha Leijnse and Barooji, {Younes Farhangi} and Arastoo, {Mohammad Reza} and Sonder, {Stine Lauritzen} and Bram Verhagen and Lena Wullkopf and Erler, {Janine Terra} and Szabolcs Semsey and Jesper Nylandsted and Oddershede, {Lene Broeng} and Amin Doostmohammadi and Bendix, {Poul Martin}",
year = "2022",
month = mar,
day = "28",
doi = "10.1038/s41467-022-28961-x",
language = "English",
volume = "13",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "nature publishing group",
number = "1",

}

RIS

TY - JOUR

T1 - Filopodia rotate and coil by actively generating twist in their actin shaft

AU - Leijnse, Natascha

AU - Barooji, Younes Farhangi

AU - Arastoo, Mohammad Reza

AU - Sonder, Stine Lauritzen

AU - Verhagen, Bram

AU - Wullkopf, Lena

AU - Erler, Janine Terra

AU - Semsey, Szabolcs

AU - Nylandsted, Jesper

AU - Oddershede, Lene Broeng

AU - Doostmohammadi, Amin

AU - Bendix, Poul Martin

PY - 2022/3/28

Y1 - 2022/3/28

N2 - The authors show how tubular surface structures in all cell types, have the ability to twist and perform rotary sweeping motion to explore the extracellular environment. This has implications for migration, sensing and cell communication.Filopodia are actin-rich structures, present on the surface of eukaryotic cells. These structures play a pivotal role by allowing cells to explore their environment, generate mechanical forces or perform chemical signaling. Their complex dynamics includes buckling, pulling, length and shape changes. We show that filopodia additionally explore their 3D extracellular space by combining growth and shrinking with axial twisting and buckling. Importantly, the actin core inside filopodia performs a twisting or spinning motion which is observed for a range of cell types spanning from earliest development to highly differentiated tissue cells. Non-equilibrium physical modeling of actin and myosin confirm that twist is an emergent phenomenon of active filaments confined in a narrow channel which is supported by measured traction forces and helical buckles that can be ascribed to accumulation of sufficient twist. These results lead us to conclude that activity induced twisting of the actin shaft is a general mechanism underlying fundamental functions of filopodia.

AB - The authors show how tubular surface structures in all cell types, have the ability to twist and perform rotary sweeping motion to explore the extracellular environment. This has implications for migration, sensing and cell communication.Filopodia are actin-rich structures, present on the surface of eukaryotic cells. These structures play a pivotal role by allowing cells to explore their environment, generate mechanical forces or perform chemical signaling. Their complex dynamics includes buckling, pulling, length and shape changes. We show that filopodia additionally explore their 3D extracellular space by combining growth and shrinking with axial twisting and buckling. Importantly, the actin core inside filopodia performs a twisting or spinning motion which is observed for a range of cell types spanning from earliest development to highly differentiated tissue cells. Non-equilibrium physical modeling of actin and myosin confirm that twist is an emergent phenomenon of active filaments confined in a narrow channel which is supported by measured traction forces and helical buckles that can be ascribed to accumulation of sufficient twist. These results lead us to conclude that activity induced twisting of the actin shaft is a general mechanism underlying fundamental functions of filopodia.

KW - EMBRYONIC STEM-CELLS

KW - GROUND-STATE

KW - DYNAMICS

KW - CYTOSKELETON

KW - ADHESION

KW - FASCIN

KW - INVADOPODIA

KW - RETRACTION

KW - MAINTAINS

KW - MIGRATION

U2 - 10.1038/s41467-022-28961-x

DO - 10.1038/s41467-022-28961-x

M3 - Journal article

C2 - 35347113

VL - 13

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

IS - 1

M1 - 1636

ER -

ID: 303445738