Sall4 and Myocd Empower Direct Cardiac Reprogramming From Adult Cardiac Fibroblasts After Injury

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Sall4 and Myocd Empower Direct Cardiac Reprogramming From Adult Cardiac Fibroblasts After Injury. / Zhao, Hong; Zhang, Yi; Xu, Xiaochan; Sun, Qiushi; Yang, Chunyan; Wang, Hao; Yang, Junbo; Yang, Yang; Yang, Xiaochun; Liu, Yi; Zhao, Yang.

I: Frontiers in Cell and Developmental Biology, Bind 9, 608367, 26.02.2021.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Zhao, H, Zhang, Y, Xu, X, Sun, Q, Yang, C, Wang, H, Yang, J, Yang, Y, Yang, X, Liu, Y & Zhao, Y 2021, 'Sall4 and Myocd Empower Direct Cardiac Reprogramming From Adult Cardiac Fibroblasts After Injury', Frontiers in Cell and Developmental Biology, bind 9, 608367. https://doi.org/10.3389/fcell.2021.608367

APA

Zhao, H., Zhang, Y., Xu, X., Sun, Q., Yang, C., Wang, H., Yang, J., Yang, Y., Yang, X., Liu, Y., & Zhao, Y. (2021). Sall4 and Myocd Empower Direct Cardiac Reprogramming From Adult Cardiac Fibroblasts After Injury. Frontiers in Cell and Developmental Biology, 9, [608367]. https://doi.org/10.3389/fcell.2021.608367

Vancouver

Zhao H, Zhang Y, Xu X, Sun Q, Yang C, Wang H o.a. Sall4 and Myocd Empower Direct Cardiac Reprogramming From Adult Cardiac Fibroblasts After Injury. Frontiers in Cell and Developmental Biology. 2021 feb. 26;9. 608367. https://doi.org/10.3389/fcell.2021.608367

Author

Zhao, Hong ; Zhang, Yi ; Xu, Xiaochan ; Sun, Qiushi ; Yang, Chunyan ; Wang, Hao ; Yang, Junbo ; Yang, Yang ; Yang, Xiaochun ; Liu, Yi ; Zhao, Yang. / Sall4 and Myocd Empower Direct Cardiac Reprogramming From Adult Cardiac Fibroblasts After Injury. I: Frontiers in Cell and Developmental Biology. 2021 ; Bind 9.

Bibtex

@article{5660059c57b54e24a603987a799a3ee8,
title = "Sall4 and Myocd Empower Direct Cardiac Reprogramming From Adult Cardiac Fibroblasts After Injury",
abstract = "Direct conversion of fibroblasts into induced cardiomyocytes (iCMs) holds promising potential to generate functional cardiomyocytes for drug development and clinical applications, especially for direct in situ heart regeneration by delivery of reprogramming genes into adult cardiac fibroblasts in injured hearts. For a decade, many cocktails of transcription factors have been developed to generate iCMs from fibroblasts of different tissues in vitro and some were applied in vivo. Here, we aimed to develop genetic cocktails that induce cardiac reprogramming directly in cultured cardiac fibroblasts isolated from adult mice with myocardial infarction (MICFs), which could be more relevant to heart diseases. We found that the widely used genetic cocktail, Gata4, Mef2c, and Tbx5 (GMT) were inefficient in reprogramming cardiomyocytes from MICFs. In a whole well of a 12-well plate, less than 10 mCherry(+) cells (",
keywords = "myofibroblast, cardiac reprogramming, high efficiency, Sall4, Myocd, MICF",
author = "Hong Zhao and Yi Zhang and Xiaochan Xu and Qiushi Sun and Chunyan Yang and Hao Wang and Junbo Yang and Yang Yang and Xiaochun Yang and Yi Liu and Yang Zhao",
year = "2021",
month = feb,
day = "26",
doi = "10.3389/fcell.2021.608367",
language = "English",
volume = "9",
journal = "Frontiers in Cell and Developmental Biology",
issn = "2296-634X",
publisher = "Frontiers Media",

}

RIS

TY - JOUR

T1 - Sall4 and Myocd Empower Direct Cardiac Reprogramming From Adult Cardiac Fibroblasts After Injury

AU - Zhao, Hong

AU - Zhang, Yi

AU - Xu, Xiaochan

AU - Sun, Qiushi

AU - Yang, Chunyan

AU - Wang, Hao

AU - Yang, Junbo

AU - Yang, Yang

AU - Yang, Xiaochun

AU - Liu, Yi

AU - Zhao, Yang

PY - 2021/2/26

Y1 - 2021/2/26

N2 - Direct conversion of fibroblasts into induced cardiomyocytes (iCMs) holds promising potential to generate functional cardiomyocytes for drug development and clinical applications, especially for direct in situ heart regeneration by delivery of reprogramming genes into adult cardiac fibroblasts in injured hearts. For a decade, many cocktails of transcription factors have been developed to generate iCMs from fibroblasts of different tissues in vitro and some were applied in vivo. Here, we aimed to develop genetic cocktails that induce cardiac reprogramming directly in cultured cardiac fibroblasts isolated from adult mice with myocardial infarction (MICFs), which could be more relevant to heart diseases. We found that the widely used genetic cocktail, Gata4, Mef2c, and Tbx5 (GMT) were inefficient in reprogramming cardiomyocytes from MICFs. In a whole well of a 12-well plate, less than 10 mCherry(+) cells (

AB - Direct conversion of fibroblasts into induced cardiomyocytes (iCMs) holds promising potential to generate functional cardiomyocytes for drug development and clinical applications, especially for direct in situ heart regeneration by delivery of reprogramming genes into adult cardiac fibroblasts in injured hearts. For a decade, many cocktails of transcription factors have been developed to generate iCMs from fibroblasts of different tissues in vitro and some were applied in vivo. Here, we aimed to develop genetic cocktails that induce cardiac reprogramming directly in cultured cardiac fibroblasts isolated from adult mice with myocardial infarction (MICFs), which could be more relevant to heart diseases. We found that the widely used genetic cocktail, Gata4, Mef2c, and Tbx5 (GMT) were inefficient in reprogramming cardiomyocytes from MICFs. In a whole well of a 12-well plate, less than 10 mCherry(+) cells (

KW - myofibroblast

KW - cardiac reprogramming

KW - high efficiency

KW - Sall4

KW - Myocd

KW - MICF

U2 - 10.3389/fcell.2021.608367

DO - 10.3389/fcell.2021.608367

M3 - Journal article

C2 - 33718351

VL - 9

JO - Frontiers in Cell and Developmental Biology

JF - Frontiers in Cell and Developmental Biology

SN - 2296-634X

M1 - 608367

ER -

ID: 259825620