Sall4 and Myocd Empower Direct Cardiac Reprogramming From Adult Cardiac Fibroblasts After Injury

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Dokumenter

  • Hong Zhao
  • Yi Zhang
  • sgk681, sgk681
  • Qiushi Sun
  • Chunyan Yang
  • Hao Wang
  • Junbo Yang
  • Yang Yang
  • Xiaochun Yang
  • Yi Liu
  • Yang Zhao

Direct conversion of fibroblasts into induced cardiomyocytes (iCMs) holds promising potential to generate functional cardiomyocytes for drug development and clinical applications, especially for direct in situ heart regeneration by delivery of reprogramming genes into adult cardiac fibroblasts in injured hearts. For a decade, many cocktails of transcription factors have been developed to generate iCMs from fibroblasts of different tissues in vitro and some were applied in vivo. Here, we aimed to develop genetic cocktails that induce cardiac reprogramming directly in cultured cardiac fibroblasts isolated from adult mice with myocardial infarction (MICFs), which could be more relevant to heart diseases. We found that the widely used genetic cocktail, Gata4, Mef2c, and Tbx5 (GMT) were inefficient in reprogramming cardiomyocytes from MICFs. In a whole well of a 12-well plate, less than 10 mCherry(+) cells (

OriginalsprogEngelsk
Artikelnummer608367
TidsskriftFrontiers in Cell and Developmental Biology
Vol/bind9
Antal sider13
ISSN2296-634X
DOI
StatusUdgivet - 26 feb. 2021

ID: 259825620