The fitness cost and benefit of phase-separated protein deposits

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The fitness cost and benefit of phase-separated protein deposits. / Sanchez de Groot, Natalia; Torrent Burgas, Marc; Ravarani, Charles N.J.; Trusina, Ala; Ventura, Salvador; Babu, M. Madan.

I: Molecular Systems Biology, Bind 15, Nr. 4, e8075, 04.2019.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Sanchez de Groot, N, Torrent Burgas, M, Ravarani, CNJ, Trusina, A, Ventura, S & Babu, MM 2019, 'The fitness cost and benefit of phase-separated protein deposits', Molecular Systems Biology, bind 15, nr. 4, e8075. https://doi.org/10.15252/msb.20178075

APA

Sanchez de Groot, N., Torrent Burgas, M., Ravarani, C. N. J., Trusina, A., Ventura, S., & Babu, M. M. (2019). The fitness cost and benefit of phase-separated protein deposits. Molecular Systems Biology, 15(4), [e8075]. https://doi.org/10.15252/msb.20178075

Vancouver

Sanchez de Groot N, Torrent Burgas M, Ravarani CNJ, Trusina A, Ventura S, Babu MM. The fitness cost and benefit of phase-separated protein deposits. Molecular Systems Biology. 2019 apr;15(4). e8075. https://doi.org/10.15252/msb.20178075

Author

Sanchez de Groot, Natalia ; Torrent Burgas, Marc ; Ravarani, Charles N.J. ; Trusina, Ala ; Ventura, Salvador ; Babu, M. Madan. / The fitness cost and benefit of phase-separated protein deposits. I: Molecular Systems Biology. 2019 ; Bind 15, Nr. 4.

Bibtex

@article{0a00c9d79eca41428761812c6064a1c2,
title = "The fitness cost and benefit of phase-separated protein deposits",
abstract = "Phase separation of soluble proteins into insoluble deposits is associated with numerous diseases. However, protein deposits can also function as membrane-less compartments for many cellular processes. What are the fitness costs and benefits of forming such deposits in different conditions? Using a model protein that phase-separates into deposits, we distinguish and quantify the fitness contribution due to the loss or gain of protein function and deposit formation in yeast. The environmental condition and the cellular demand for the protein function emerge as key determinants of fitness. Protein deposit formation can influence cell-to-cell variation in free protein abundance between individuals of a cell population (i.e., gene expression noise). This results in variable manifestation of protein function and a continuous range of phenotypes in a cell population, favoring survival of some individuals in certain environments. Thus, protein deposit formation by phase separation might be a mechanism to sense protein concentration in cells and to generate phenotypic variability. The selectable phenotypic variability, previously described for prions, could be a general property of proteins that can form phase-separated assemblies and may influence cell fitness.",
keywords = "cell fitness, natural selection, phase separation, phenotypic diversity, protein deposit",
author = "{Sanchez de Groot}, Natalia and {Torrent Burgas}, Marc and Ravarani, {Charles N.J.} and Ala Trusina and Salvador Ventura and Babu, {M. Madan}",
year = "2019",
month = apr,
doi = "10.15252/msb.20178075",
language = "English",
volume = "15",
journal = "Molecular Systems Biology",
issn = "1744-4292",
publisher = "Wiley-Blackwell",
number = "4",

}

RIS

TY - JOUR

T1 - The fitness cost and benefit of phase-separated protein deposits

AU - Sanchez de Groot, Natalia

AU - Torrent Burgas, Marc

AU - Ravarani, Charles N.J.

AU - Trusina, Ala

AU - Ventura, Salvador

AU - Babu, M. Madan

PY - 2019/4

Y1 - 2019/4

N2 - Phase separation of soluble proteins into insoluble deposits is associated with numerous diseases. However, protein deposits can also function as membrane-less compartments for many cellular processes. What are the fitness costs and benefits of forming such deposits in different conditions? Using a model protein that phase-separates into deposits, we distinguish and quantify the fitness contribution due to the loss or gain of protein function and deposit formation in yeast. The environmental condition and the cellular demand for the protein function emerge as key determinants of fitness. Protein deposit formation can influence cell-to-cell variation in free protein abundance between individuals of a cell population (i.e., gene expression noise). This results in variable manifestation of protein function and a continuous range of phenotypes in a cell population, favoring survival of some individuals in certain environments. Thus, protein deposit formation by phase separation might be a mechanism to sense protein concentration in cells and to generate phenotypic variability. The selectable phenotypic variability, previously described for prions, could be a general property of proteins that can form phase-separated assemblies and may influence cell fitness.

AB - Phase separation of soluble proteins into insoluble deposits is associated with numerous diseases. However, protein deposits can also function as membrane-less compartments for many cellular processes. What are the fitness costs and benefits of forming such deposits in different conditions? Using a model protein that phase-separates into deposits, we distinguish and quantify the fitness contribution due to the loss or gain of protein function and deposit formation in yeast. The environmental condition and the cellular demand for the protein function emerge as key determinants of fitness. Protein deposit formation can influence cell-to-cell variation in free protein abundance between individuals of a cell population (i.e., gene expression noise). This results in variable manifestation of protein function and a continuous range of phenotypes in a cell population, favoring survival of some individuals in certain environments. Thus, protein deposit formation by phase separation might be a mechanism to sense protein concentration in cells and to generate phenotypic variability. The selectable phenotypic variability, previously described for prions, could be a general property of proteins that can form phase-separated assemblies and may influence cell fitness.

KW - cell fitness

KW - natural selection

KW - phase separation

KW - phenotypic diversity

KW - protein deposit

UR - http://www.scopus.com/inward/record.url?scp=85064540380&partnerID=8YFLogxK

U2 - 10.15252/msb.20178075

DO - 10.15252/msb.20178075

M3 - Journal article

C2 - 30962358

AN - SCOPUS:85064540380

VL - 15

JO - Molecular Systems Biology

JF - Molecular Systems Biology

SN - 1744-4292

IS - 4

M1 - e8075

ER -

ID: 229439895