How Membrane Geometry Regulates Protein Sorting Independently of Mean Curvature

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

How Membrane Geometry Regulates Protein Sorting Independently of Mean Curvature. / Larsen, Jannik B.; Rosholm, Kadla R.; Kennard, Celeste; Pedersen, Soren L.; Munch, Henrik K.; Tkach, Vadym; Sakon, John J.; Bjornholm, Thomas; Weninger, Keith R.; Bendix, Poul Martin; Jensen, Knud J.; Hatzakis, Nikos S.; Uline, Mark J.; Stamou, Dimitrios.

In: ACS Central Science, Vol. 6, No. 7, 2020, p. 1159-1168.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Larsen, JB, Rosholm, KR, Kennard, C, Pedersen, SL, Munch, HK, Tkach, V, Sakon, JJ, Bjornholm, T, Weninger, KR, Bendix, PM, Jensen, KJ, Hatzakis, NS, Uline, MJ & Stamou, D 2020, 'How Membrane Geometry Regulates Protein Sorting Independently of Mean Curvature', ACS Central Science, vol. 6, no. 7, pp. 1159-1168. https://doi.org/10.1021/acscentsci.0c00419

APA

Larsen, J. B., Rosholm, K. R., Kennard, C., Pedersen, S. L., Munch, H. K., Tkach, V., Sakon, J. J., Bjornholm, T., Weninger, K. R., Bendix, P. M., Jensen, K. J., Hatzakis, N. S., Uline, M. J., & Stamou, D. (2020). How Membrane Geometry Regulates Protein Sorting Independently of Mean Curvature. ACS Central Science, 6(7), 1159-1168. https://doi.org/10.1021/acscentsci.0c00419

Vancouver

Larsen JB, Rosholm KR, Kennard C, Pedersen SL, Munch HK, Tkach V et al. How Membrane Geometry Regulates Protein Sorting Independently of Mean Curvature. ACS Central Science. 2020;6(7):1159-1168. https://doi.org/10.1021/acscentsci.0c00419

Author

Larsen, Jannik B. ; Rosholm, Kadla R. ; Kennard, Celeste ; Pedersen, Soren L. ; Munch, Henrik K. ; Tkach, Vadym ; Sakon, John J. ; Bjornholm, Thomas ; Weninger, Keith R. ; Bendix, Poul Martin ; Jensen, Knud J. ; Hatzakis, Nikos S. ; Uline, Mark J. ; Stamou, Dimitrios. / How Membrane Geometry Regulates Protein Sorting Independently of Mean Curvature. In: ACS Central Science. 2020 ; Vol. 6, No. 7. pp. 1159-1168.

Bibtex

@article{2a0682c95f914b73975801fdaa5c9645,
title = "How Membrane Geometry Regulates Protein Sorting Independently of Mean Curvature",
abstract = "Biological membranes have distinct geometries that confer specific functions. However, the molecular mechanisms underlying the phenomenological geometry/function correlations remain elusive. We studied the effect of membrane geometry on the localization of membrane-bound proteins. Quantitative comparative experiments between the two most abundant cellular membrane geometries, spherical and cylindrical, revealed that geometry regulates the spatial segregation of proteins. The measured geometry-driven segregation reached 50-fold for membranes of the same mean curvature, demonstrating a crucial and hitherto unaccounted contribution by Gaussian curvature. Molecular-field theory calculations elucidated the underlying physical and molecular mechanisms. Our results reveal that distinct membrane geometries have specific physicochemical properties and thus establish a ubiquitous mechanistic foundation for unravelling the conserved correlations between biological function and membrane polymorphism.",
keywords = "AMPHIPATHIC HELICES, SYNAPTOTAGMIN, MECHANISMS, LOCALIZATION, AMPHIPHYSIN, INDUCE, DOMAIN, CELL",
author = "Larsen, {Jannik B.} and Rosholm, {Kadla R.} and Celeste Kennard and Pedersen, {Soren L.} and Munch, {Henrik K.} and Vadym Tkach and Sakon, {John J.} and Thomas Bjornholm and Weninger, {Keith R.} and Bendix, {Poul Martin} and Jensen, {Knud J.} and Hatzakis, {Nikos S.} and Uline, {Mark J.} and Dimitrios Stamou",
year = "2020",
doi = "10.1021/acscentsci.0c00419",
language = "English",
volume = "6",
pages = "1159--1168",
journal = "A C S Central Science",
issn = "2374-7943",
publisher = "American Chemical Society",
number = "7",

}

RIS

TY - JOUR

T1 - How Membrane Geometry Regulates Protein Sorting Independently of Mean Curvature

AU - Larsen, Jannik B.

AU - Rosholm, Kadla R.

AU - Kennard, Celeste

AU - Pedersen, Soren L.

AU - Munch, Henrik K.

AU - Tkach, Vadym

AU - Sakon, John J.

AU - Bjornholm, Thomas

AU - Weninger, Keith R.

AU - Bendix, Poul Martin

AU - Jensen, Knud J.

AU - Hatzakis, Nikos S.

AU - Uline, Mark J.

AU - Stamou, Dimitrios

PY - 2020

Y1 - 2020

N2 - Biological membranes have distinct geometries that confer specific functions. However, the molecular mechanisms underlying the phenomenological geometry/function correlations remain elusive. We studied the effect of membrane geometry on the localization of membrane-bound proteins. Quantitative comparative experiments between the two most abundant cellular membrane geometries, spherical and cylindrical, revealed that geometry regulates the spatial segregation of proteins. The measured geometry-driven segregation reached 50-fold for membranes of the same mean curvature, demonstrating a crucial and hitherto unaccounted contribution by Gaussian curvature. Molecular-field theory calculations elucidated the underlying physical and molecular mechanisms. Our results reveal that distinct membrane geometries have specific physicochemical properties and thus establish a ubiquitous mechanistic foundation for unravelling the conserved correlations between biological function and membrane polymorphism.

AB - Biological membranes have distinct geometries that confer specific functions. However, the molecular mechanisms underlying the phenomenological geometry/function correlations remain elusive. We studied the effect of membrane geometry on the localization of membrane-bound proteins. Quantitative comparative experiments between the two most abundant cellular membrane geometries, spherical and cylindrical, revealed that geometry regulates the spatial segregation of proteins. The measured geometry-driven segregation reached 50-fold for membranes of the same mean curvature, demonstrating a crucial and hitherto unaccounted contribution by Gaussian curvature. Molecular-field theory calculations elucidated the underlying physical and molecular mechanisms. Our results reveal that distinct membrane geometries have specific physicochemical properties and thus establish a ubiquitous mechanistic foundation for unravelling the conserved correlations between biological function and membrane polymorphism.

KW - AMPHIPATHIC HELICES

KW - SYNAPTOTAGMIN

KW - MECHANISMS

KW - LOCALIZATION

KW - AMPHIPHYSIN

KW - INDUCE

KW - DOMAIN

KW - CELL

U2 - 10.1021/acscentsci.0c00419

DO - 10.1021/acscentsci.0c00419

M3 - Journal article

C2 - 32724850

VL - 6

SP - 1159

EP - 1168

JO - A C S Central Science

JF - A C S Central Science

SN - 2374-7943

IS - 7

ER -

ID: 247155911