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Tian Wang and Thomas Heimburg
Niels Bohr Institute, University of Copenhagen.


Title: Effect of Anesthetics on Action Potential Propagation.

Abstract: Local anesthesia has been attributed to the specific interaction of local anesthetics with (sodium) channel proteins, while the action of general anesthetics still remains unclear. However, already at the beginning of 20th century Meyer and Overton independently found that the critical anesthetic dose of anesthetics, regardless of their types, is linearly proportional to their solubility in olive oil. Heimburg and Jackson proposed that the action potential is a density pulse (soliton) propagating in biological membranes and made a thermodynamic extension of the Meyer-Overton rule, incorporating the effects of melting point depression that originates from van't Hoff's law. In this work, experiments were done on nerves to study the effect of local anesthetic lidocaine on compound action potential and action potential from a single neuron. The experimental data were then compared with the simulation results by solving the soliton equations in 1D cylindrical membrane with lidocaine inside the system. Anesthetics move the chain melting transition temperature of membranes far away from the physiological temperature, thus it requires a higher free energy to induce the phase transition, resulting in a higher stimulation voltage to reach the maximum amplitude of the action potential. At the same time, the velocity of action potential propagation is slightly slowed down by lidocaine.

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