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Mathias Winterhalter

Jacobs University Bremen

Title: Permeation of small hydrophilic molecules across bacterial membranes.

Abstract: The outer cell wall of Gram-negative bacteria contains a number of channel forming proteins called porins. Such channels allow e.g. bacteria to harvest water soluble nutrients. Here we present our current approach to quantify the uptake. Single porins are reconstituted into planar lipid membranes and characterized by time resolved ion current. Molecules diffusing into the channel will create typical fluctuations in ion conductance. However the analysis is not sufficient to quantify transport.

Literature:
Prathyusha BS, et al. Role of electroosmosis in the permeation of neutral molecules in electrophysiology: The example of cyclodextrin and CymA. Biophys. J. 110 (2016) 600-11.
Bajaj H, et al. Molecular Basis of Filtering Carbapenems by Porins from ‰-Lactam-resistant Clinical Strains of Escherichia coli; J. Biol. Chem. 291 (2016) 2837-47.

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