Cyclic-di-GMP signaling controls metabolic activity in Pseudomonas aeruginosa
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Cyclic-di-GMP signaling controls metabolic activity in Pseudomonas aeruginosa. / Lichtenberg, Mads; Kragh, Kasper Nørskov; Fritz, Blaine; Kirkegaard, Julius B.; Tolker-Nielsen, Tim; Bjarnsholt, Thomas.
I: Cell Reports, Bind 41, Nr. 3, 2022, s. 111515.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Cyclic-di-GMP signaling controls metabolic activity in Pseudomonas aeruginosa
AU - Lichtenberg, Mads
AU - Kragh, Kasper Nørskov
AU - Fritz, Blaine
AU - Kirkegaard, Julius B.
AU - Tolker-Nielsen, Tim
AU - Bjarnsholt, Thomas
N1 - Publisher Copyright: Copyright © 2022 The Author(s). Published by Elsevier Inc. All rights reserved.
PY - 2022
Y1 - 2022
N2 - Bacteria in biofilms are embedded in extracellular matrix and display low metabolic activity, partly due to insufficient diffusive exchange of metabolic substrate. The extracellular matrix and low metabolic activity both contribute to the high antibiotic tolerance-the hallmark of biofilm bacteria. The second messenger molecule, c-di-GMP, regulates biofilm development in Pseudomonas aeruginosa, where high internal levels lead to biofilm formation and low levels are associated with planktonic bacteria. Using a microcalorimetric approach, we show that c-di-GMP signaling is a major determinant of the metabolic activity of P. aeruginosa, both in planktonic culture and in two biofilm models. The high c-di-GMP content of biofilm bacteria forces them to rapidly spend a large amount of energy on the production of exopolysaccharides, resulting in a subsequent low metabolic state. This suggests that the low metabolic state of bacteria in mature biofilms, to some extent, is a consequence of a c-di-GMP-regulated survival strategy.
AB - Bacteria in biofilms are embedded in extracellular matrix and display low metabolic activity, partly due to insufficient diffusive exchange of metabolic substrate. The extracellular matrix and low metabolic activity both contribute to the high antibiotic tolerance-the hallmark of biofilm bacteria. The second messenger molecule, c-di-GMP, regulates biofilm development in Pseudomonas aeruginosa, where high internal levels lead to biofilm formation and low levels are associated with planktonic bacteria. Using a microcalorimetric approach, we show that c-di-GMP signaling is a major determinant of the metabolic activity of P. aeruginosa, both in planktonic culture and in two biofilm models. The high c-di-GMP content of biofilm bacteria forces them to rapidly spend a large amount of energy on the production of exopolysaccharides, resulting in a subsequent low metabolic state. This suggests that the low metabolic state of bacteria in mature biofilms, to some extent, is a consequence of a c-di-GMP-regulated survival strategy.
KW - biofilm
KW - c-di-GMP
KW - CP: Microbiology
KW - exopolysaccharides
KW - matrix
KW - metabolism
KW - microcalorimetry
KW - Pseudomonas aeruginosa
U2 - 10.1016/j.celrep.2022.111515
DO - 10.1016/j.celrep.2022.111515
M3 - Journal article
C2 - 36260996
AN - SCOPUS:85140415314
VL - 41
SP - 111515
JO - Cell Reports
JF - Cell Reports
SN - 2211-1247
IS - 3
ER -
ID: 324317736