DNA supercoiling enhances cooperativity and efficiency of an epigenetic switch
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DNA supercoiling enhances cooperativity and efficiency of an epigenetic switch. / Norregaard, Kamilla; Andersson, Magnus; Sneppen, Kim; Nielsen, Peter Eigil; Brown, Stanley; Oddershede, Lene B.
I: Proceedings of the National Academy of Sciences USA (PNAS), Bind 110, Nr. 43, 22.10.2013, s. 17386-91.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - DNA supercoiling enhances cooperativity and efficiency of an epigenetic switch
AU - Norregaard, Kamilla
AU - Andersson, Magnus
AU - Sneppen, Kim
AU - Nielsen, Peter Eigil
AU - Brown, Stanley
AU - Oddershede, Lene B
PY - 2013/10/22
Y1 - 2013/10/22
N2 - Bacteriophage λ stably maintains its dormant prophage state but efficiently enters lytic development in response to DNA damage. The mediator of these processes is the λ repressor protein, CI, and its interactions with λ operator DNA. This λ switch is a model on the basis of which epigenetic switch regulation is understood. Using single molecule analysis, we directly examined the stability of the CI-operator structure in its natural, supercoiled state. We marked positions adjacent to the λ operators with peptide nucleic acids and monitored their movement by tethered particle tracking. Compared with relaxed DNA, the presence of supercoils greatly enhances juxtaposition probability. Also, the efficiency and cooperativity of the λ switch is significantly increased in the supercoiled system compared with a linear assay, increasing the Hill coefficient.
AB - Bacteriophage λ stably maintains its dormant prophage state but efficiently enters lytic development in response to DNA damage. The mediator of these processes is the λ repressor protein, CI, and its interactions with λ operator DNA. This λ switch is a model on the basis of which epigenetic switch regulation is understood. Using single molecule analysis, we directly examined the stability of the CI-operator structure in its natural, supercoiled state. We marked positions adjacent to the λ operators with peptide nucleic acids and monitored their movement by tethered particle tracking. Compared with relaxed DNA, the presence of supercoils greatly enhances juxtaposition probability. Also, the efficiency and cooperativity of the λ switch is significantly increased in the supercoiled system compared with a linear assay, increasing the Hill coefficient.
KW - Algorithms
KW - Bacteriophage lambda
KW - DNA, Superhelical
KW - DNA, Viral
KW - Epigenesis, Genetic
KW - Lysogeny
KW - Models, Genetic
KW - Operator Regions, Genetic
KW - Prophages
KW - Protein Binding
KW - Repressor Proteins
KW - Thermodynamics
KW - Viral Regulatory and Accessory Proteins
KW - Virus Activation
U2 - 10.1073/pnas.1215907110
DO - 10.1073/pnas.1215907110
M3 - Journal article
C2 - 24101469
VL - 110
SP - 17386
EP - 17391
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 43
ER -
ID: 77591206