Dynamic features of gene expression control by small regulatory RNAs
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Dynamic features of gene expression control by small regulatory RNAs. / Mitarai, Namiko; Benjamin, Julie-Anna M; Krishna, Sandeep; Semsey, Szabolcs; Csiszovszki, Zsolt; Massé, Eric; Sneppen, Kim.
I: Proceedings of the National Academy of Sciences of the United States of America, Bind 106, Nr. 26, 30.06.2009, s. 10655-9.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Dynamic features of gene expression control by small regulatory RNAs
AU - Mitarai, Namiko
AU - Benjamin, Julie-Anna M
AU - Krishna, Sandeep
AU - Semsey, Szabolcs
AU - Csiszovszki, Zsolt
AU - Massé, Eric
AU - Sneppen, Kim
PY - 2009/6/30
Y1 - 2009/6/30
N2 - Small regulatory RNAs (sRNAs) in eukaryotes and bacteria play an important role in the regulation of gene expression either by binding to regulatory proteins or directly to target mRNAs. Two of the best-characterized bacterial sRNAs, Spot42 and RyhB, form a complementary pair with the ribosome binding region of their target mRNAs, thereby inhibiting translation or promoting mRNA degradation. To investigate the steady-state and dynamic potential of such sRNAs, we examine the 2 key parameters characterizing sRNA regulation: the capacity to overexpress the sRNA relative to its target mRNA and the speed at which the target mRNA is irreversibly inactivated. We demonstrate different methods to determine these 2 key parameters, for Spot42 and RyhB, which combine biochemical and genetic experiments with computational analysis. We have developed a mathematical model that describes the functional properties of sRNAs with various characteristic parameters. We observed that Spot42 and RyhB function in distinctive parameter regimes, which result in divergent mechanisms.
AB - Small regulatory RNAs (sRNAs) in eukaryotes and bacteria play an important role in the regulation of gene expression either by binding to regulatory proteins or directly to target mRNAs. Two of the best-characterized bacterial sRNAs, Spot42 and RyhB, form a complementary pair with the ribosome binding region of their target mRNAs, thereby inhibiting translation or promoting mRNA degradation. To investigate the steady-state and dynamic potential of such sRNAs, we examine the 2 key parameters characterizing sRNA regulation: the capacity to overexpress the sRNA relative to its target mRNA and the speed at which the target mRNA is irreversibly inactivated. We demonstrate different methods to determine these 2 key parameters, for Spot42 and RyhB, which combine biochemical and genetic experiments with computational analysis. We have developed a mathematical model that describes the functional properties of sRNAs with various characteristic parameters. We observed that Spot42 and RyhB function in distinctive parameter regimes, which result in divergent mechanisms.
KW - Algorithms
KW - Bacterial Proteins
KW - Gene Expression Regulation, Bacterial
KW - Models, Genetic
KW - RNA, Bacterial
KW - RNA, Messenger
KW - RNA, Untranslated
KW - Transcription, Genetic
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
U2 - 10.1073/pnas.0901466106
DO - 10.1073/pnas.0901466106
M3 - Journal article
C2 - 19541626
VL - 106
SP - 10655
EP - 10659
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 26
ER -
ID: 163917323