Protocol to assess metabolic activity of Pseudomonas aeruginosa by measuring heat flow using isothermal calorimetry
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Protocol to assess metabolic activity of Pseudomonas aeruginosa by measuring heat flow using isothermal calorimetry. / Beilharz, Katrin; Kragh, Kasper Nørskov; Fritz, Blaine; Kirkegaard, Julius B.; Tolker-Nielsen, Tim; Bjarnsholt, Thomas; Lichtenberg, Mads.
I: STAR Protocols, Bind 4, Nr. 2, 102269, 2023.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Protocol to assess metabolic activity of Pseudomonas aeruginosa by measuring heat flow using isothermal calorimetry
AU - Beilharz, Katrin
AU - Kragh, Kasper Nørskov
AU - Fritz, Blaine
AU - Kirkegaard, Julius B.
AU - Tolker-Nielsen, Tim
AU - Bjarnsholt, Thomas
AU - Lichtenberg, Mads
N1 - Publisher Copyright: © 2023 The Authors
PY - 2023
Y1 - 2023
N2 - Here, we present a protocol for assessing metabolic activity of bacterial populations by measuring heat flow using isothermal calorimetry. We outline the steps for preparing the different growth models of Pseudomonas aeruginosa and performing continuous metabolic activity measurements in the calScreener. We detail simple principal component analysis to differentiate between metabolic states of different populations and probabilistic logistic classification to assess resemblance to wild-type bacteria. This protocol for fine-scale metabolic measurement can aid in understanding microbial physiology. For complete details on the use and execution of this protocol, please refer to Lichtenberg et al. (2022).1
AB - Here, we present a protocol for assessing metabolic activity of bacterial populations by measuring heat flow using isothermal calorimetry. We outline the steps for preparing the different growth models of Pseudomonas aeruginosa and performing continuous metabolic activity measurements in the calScreener. We detail simple principal component analysis to differentiate between metabolic states of different populations and probabilistic logistic classification to assess resemblance to wild-type bacteria. This protocol for fine-scale metabolic measurement can aid in understanding microbial physiology. For complete details on the use and execution of this protocol, please refer to Lichtenberg et al. (2022).1
KW - Cell-based Assays
KW - Metabolism
KW - Microbiology
U2 - 10.1016/j.xpro.2023.102269
DO - 10.1016/j.xpro.2023.102269
M3 - Journal article
C2 - 37133990
AN - SCOPUS:85153888219
VL - 4
JO - STAR Protocols
JF - STAR Protocols
SN - 2666-1667
IS - 2
M1 - 102269
ER -
ID: 346204389