Synthetic Membrane Shaper for Controlled Liposome Deformation

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Standard

Synthetic Membrane Shaper for Controlled Liposome Deformation. / De Franceschi, Nicola; Pezeshkian, Weria; Fragasso, Alessio; Bruininks, Bart M H; Tsai, Sean; Marrink, Siewert J; Dekker, Cees.

I: ACS Nano, Bind 17, 28.11.2022, s. 966-978.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

De Franceschi, N, Pezeshkian, W, Fragasso, A, Bruininks, BMH, Tsai, S, Marrink, SJ & Dekker, C 2022, 'Synthetic Membrane Shaper for Controlled Liposome Deformation', ACS Nano, bind 17, s. 966-978. https://doi.org/10.1021/acsnano.2c06125

APA

De Franceschi, N., Pezeshkian, W., Fragasso, A., Bruininks, B. M. H., Tsai, S., Marrink, S. J., & Dekker, C. (2022). Synthetic Membrane Shaper for Controlled Liposome Deformation. ACS Nano, 17, 966-978. https://doi.org/10.1021/acsnano.2c06125

Vancouver

De Franceschi N, Pezeshkian W, Fragasso A, Bruininks BMH, Tsai S, Marrink SJ o.a. Synthetic Membrane Shaper for Controlled Liposome Deformation. ACS Nano. 2022 nov. 28;17:966-978. https://doi.org/10.1021/acsnano.2c06125

Author

De Franceschi, Nicola ; Pezeshkian, Weria ; Fragasso, Alessio ; Bruininks, Bart M H ; Tsai, Sean ; Marrink, Siewert J ; Dekker, Cees. / Synthetic Membrane Shaper for Controlled Liposome Deformation. I: ACS Nano. 2022 ; Bind 17. s. 966-978.

Bibtex

@article{0e81cc07ca8944b49d88ebd8e3514de0,
title = "Synthetic Membrane Shaper for Controlled Liposome Deformation",
abstract = "Shape defines the structure and function of cellular membranes. In cell division, the cell membrane deforms into a {"}dumbbell{"} shape, while organelles such as the autophagosome exhibit {"}stomatocyte{"} shapes. Bottom-up in vitro reconstitution of protein machineries that stabilize or resolve the membrane necks in such deformed liposome structures is of considerable interest to characterize their function. Here we develop a DNA-nanotechnology-based approach that we call the synthetic membrane shaper (SMS), where cholesterol-linked DNA structures attach to the liposome membrane to reproducibly generate high yields of stomatocytes and dumbbells. In silico simulations confirm the shape-stabilizing role of the SMS. We show that the SMS is fully compatible with protein reconstitution by assembling bacterial divisome proteins (DynaminA, FtsZ:ZipA) at the catenoidal neck of these membrane structures. The SMS approach provides a general tool for studying protein binding to complex membrane geometries that will greatly benefit synthetic cell research.",
author = "{De Franceschi}, Nicola and Weria Pezeshkian and Alessio Fragasso and Bruininks, {Bart M H} and Sean Tsai and Marrink, {Siewert J} and Cees Dekker",
year = "2022",
month = nov,
day = "28",
doi = "10.1021/acsnano.2c06125",
language = "English",
volume = "17",
pages = "966--978",
journal = "A C S Nano",
issn = "1936-0851",
publisher = "American Chemical Society",

}

RIS

TY - JOUR

T1 - Synthetic Membrane Shaper for Controlled Liposome Deformation

AU - De Franceschi, Nicola

AU - Pezeshkian, Weria

AU - Fragasso, Alessio

AU - Bruininks, Bart M H

AU - Tsai, Sean

AU - Marrink, Siewert J

AU - Dekker, Cees

PY - 2022/11/28

Y1 - 2022/11/28

N2 - Shape defines the structure and function of cellular membranes. In cell division, the cell membrane deforms into a "dumbbell" shape, while organelles such as the autophagosome exhibit "stomatocyte" shapes. Bottom-up in vitro reconstitution of protein machineries that stabilize or resolve the membrane necks in such deformed liposome structures is of considerable interest to characterize their function. Here we develop a DNA-nanotechnology-based approach that we call the synthetic membrane shaper (SMS), where cholesterol-linked DNA structures attach to the liposome membrane to reproducibly generate high yields of stomatocytes and dumbbells. In silico simulations confirm the shape-stabilizing role of the SMS. We show that the SMS is fully compatible with protein reconstitution by assembling bacterial divisome proteins (DynaminA, FtsZ:ZipA) at the catenoidal neck of these membrane structures. The SMS approach provides a general tool for studying protein binding to complex membrane geometries that will greatly benefit synthetic cell research.

AB - Shape defines the structure and function of cellular membranes. In cell division, the cell membrane deforms into a "dumbbell" shape, while organelles such as the autophagosome exhibit "stomatocyte" shapes. Bottom-up in vitro reconstitution of protein machineries that stabilize or resolve the membrane necks in such deformed liposome structures is of considerable interest to characterize their function. Here we develop a DNA-nanotechnology-based approach that we call the synthetic membrane shaper (SMS), where cholesterol-linked DNA structures attach to the liposome membrane to reproducibly generate high yields of stomatocytes and dumbbells. In silico simulations confirm the shape-stabilizing role of the SMS. We show that the SMS is fully compatible with protein reconstitution by assembling bacterial divisome proteins (DynaminA, FtsZ:ZipA) at the catenoidal neck of these membrane structures. The SMS approach provides a general tool for studying protein binding to complex membrane geometries that will greatly benefit synthetic cell research.

U2 - 10.1021/acsnano.2c06125

DO - 10.1021/acsnano.2c06125

M3 - Journal article

C2 - 36441529

VL - 17

SP - 966

EP - 978

JO - A C S Nano

JF - A C S Nano

SN - 1936-0851

ER -

ID: 328042263