TY - JOUR

T1 - Global Spore Sampling Project

T2 - A global, standardized dataset of airborne fungal DNA

AU - Ovaskainen, Otso

AU - Abrego, Nerea

AU - Furneaux, Brendan

AU - Hardwick, Bess

AU - Somervuo, Panu

AU - Palorinne, Isabella

AU - Andrew, Nigel R

AU - Babiy, Ulyana V

AU - Bao, Tan

AU - Bazzano, Gisela

AU - Bondarchuk, Svetlana N

AU - Bonebrake, Timothy C

AU - Brennan, Georgina L

AU - Bret-Harte, Syndonia

AU - Bässler, Claus

AU - Cagnolo, Luciano

AU - Cameron, Erin K

AU - Chapurlat, Elodie

AU - Creer, Simon

AU - D'Acqui, Luigi P

AU - de Vere, Natasha

AU - Desprez-Loustau, Marie-Laure

AU - Dongmo, Michel A K

AU - Dyrholm Jacobsen, Ida B

AU - Fisher, Brian L

AU - Flores de Jesus, Miguel

AU - Gilbert, Gregory S

AU - Griffith, Gareth W

AU - Gritsuk, Anna A

AU - Gross, Andrin

AU - Grudd, Håkan

AU - Halme, Panu

AU - Hanna, Rachid

AU - Hansen, Jannik

AU - Hansen, Lars Holst

AU - Hegbe, Apollon D M T

AU - Hill, Sarah

AU - Hogg, Ian D

AU - Hultman, Jenni

AU - Hyde, Kevin D

AU - Hynson, Nicole A

AU - Ivanova, Natalia

AU - Karisto, Petteri

AU - Kerdraon, Deirdre

AU - Knorre, Anastasia

AU - Krisai-Greilhuber, Irmgard

AU - Kurhinen, Juri

AU - Kuzmina, Masha

AU - Lecomte, Nicolas

AU - Lecomte, Erin

AU - Loaiza, Viviana

AU - Lundin, Erik

AU - Meire, Alexander

AU - Mešić, Armin

AU - Miettinen, Otto

AU - Monkhause, Norman

AU - Mortimer, Peter

AU - Müller, Jörg

AU - Nilsson, R Henrik

AU - Nonti, Puani Yannick C

AU - Nordén, Jenni

AU - Nordén, Björn

AU - Paz, Claudia

AU - Pellikka, Petri

AU - Pereira, Danilo

AU - Petch, Geoff

AU - Pitkänen, Juha-Matti

AU - Popa, Flavius

AU - Potter, Caitlin

AU - Purhonen, Jenna

AU - Pätsi, Sanna

AU - Rafiq, Abdullah

AU - Raharinjanahary, Dimby

AU - Rakos, Niklas

AU - Rathnayaka, Achala R

AU - Raundrup, Katrine

AU - Rebriev, Yury A

AU - Rikkinen, Jouko

AU - Rogers, Hanna M K

AU - Rogovsky, Andrey

AU - Rozhkov, Yuri

AU - Runnel, Kadri

AU - Saarto, Annika

AU - Savchenko, Anton

AU - Schlegel, Markus

AU - Schmidt, Niels Martin

AU - Seibold, Sebastian

AU - Skjøth, Carsten

AU - Stengel, Elisa

AU - Sutyrina, Svetlana V

AU - Syvänperä, Ilkka

AU - Tedersoo, Leho

AU - Timm, Jebidiah

AU - Tipton, Laura

AU - Toju, Hirokazu

AU - Uscka-Perzanowska, Maria

AU - van der Bank, Michelle

AU - Herman van der Bank, F

AU - Vandenbrink, Bryan

AU - Ventura, Stefano

AU - Vignisson, Solvi R

AU - Wang, Xiaoyang

AU - Weisser, Wolfgang W

AU - Wijesinghe, Subodini N

AU - Joseph Wright, S

AU - Yang, Chunyan

AU - Yorou, Nourou S

AU - Young, Amanda

AU - Yu, Douglas W

AU - Zakharov, Evgeny V

AU - Hebert, Paul D N

AU - Roslin, Tomas

N1 - © 2024. The Author(s).

PY - 2024/5/30

Y1 - 2024/5/30

N2 - Novel methods for sampling and characterizing biodiversity hold great promise for re-evaluating patterns of life across the planet. The sampling of airborne spores with a cyclone sampler, and the sequencing of their DNA, have been suggested as an efficient and well-calibrated tool for surveying fungal diversity across various environments. Here we present data originating from the Global Spore Sampling Project, comprising 2,768 samples collected during two years at 47 outdoor locations across the world. Each sample represents fungal DNA extracted from 24 m 3 of air. We applied a conservative bioinformatics pipeline that filtered out sequences that did not show strong evidence of representing a fungal species. The pipeline yielded 27,954 species-level operational taxonomic units (OTUs). Each OTU is accompanied by a probabilistic taxonomic classification, validated through comparison with expert evaluations. To examine the potential of the data for ecological analyses, we partitioned the variation in species distributions into spatial and seasonal components, showing a strong effect of the annual mean temperature on community composition.

AB - Novel methods for sampling and characterizing biodiversity hold great promise for re-evaluating patterns of life across the planet. The sampling of airborne spores with a cyclone sampler, and the sequencing of their DNA, have been suggested as an efficient and well-calibrated tool for surveying fungal diversity across various environments. Here we present data originating from the Global Spore Sampling Project, comprising 2,768 samples collected during two years at 47 outdoor locations across the world. Each sample represents fungal DNA extracted from 24 m 3 of air. We applied a conservative bioinformatics pipeline that filtered out sequences that did not show strong evidence of representing a fungal species. The pipeline yielded 27,954 species-level operational taxonomic units (OTUs). Each OTU is accompanied by a probabilistic taxonomic classification, validated through comparison with expert evaluations. To examine the potential of the data for ecological analyses, we partitioned the variation in species distributions into spatial and seasonal components, showing a strong effect of the annual mean temperature on community composition.

KW - Air Microbiology

KW - Spores, Fungal

KW - DNA, Fungal/analysis

KW - Fungi/genetics

KW - Biodiversity

U2 - 10.1038/s41597-024-03410-0

DO - 10.1038/s41597-024-03410-0

M3 - Journal article

C2 - 38816458

VL - 11

SP - 561

JO - Scientific data

JF - Scientific data

SN - 2052-4463

IS - 1

ER -