Immunostaining protocol for infiltrating brain cancer spheroids for light-sheet imaging
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Documents
- journal.pone.0281161
Final published version, 1.49 MB, PDF document
Glioblastoma tumors form in brains' white matter and are fast-growing and aggressive. Poor prognosis is the result of therapeutic resistance and infiltrating growth into the surrounding brain. Here we present a protocol for the detection of the cytoskeleton intermediate filament, vimentin, in cells at the proliferating spheroid surface. By combining a classical invasion assay with immunofluorescence and light-sheet imaging, we find that it is exactly these cytoskeleton-reinforcing cells on the spheroid's surface that will start the infiltration. We anticipate our results to be the starting point of more sophisticated investigation of anti-cancer drug effects on cytoskeleton reorganisation.
Original language | English |
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Article number | 0281161 |
Journal | PLoS ONE |
Volume | 18 |
Issue number | 2 |
Number of pages | 8 |
ISSN | 1932-6203 |
DOIs | |
Publication status | Published - 9 Feb 2023 |
- TO-MESENCHYMAL TRANSITION, INTERMEDIATE-FILAMENTS, VIMENTIN, INVASION
Research areas
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