Prevention of H2O2-induced oxidative stress in Chang liver cells by 4-hydroxybenzyl-chitooligomers
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Prevention of H2O2-induced oxidative stress in Chang liver cells by 4-hydroxybenzyl-chitooligomers. / Trinh, Mai Duy Luu; Ngo, Dai-Hung; Tran, Dang-Khoa; Tran, Quoc-Tuan; Vo, Thanh-Sang; Dinh, Minh-Hiep; Ngo, Dai-Nghiep.
In: Carbohydrate Polymers, Vol. 103, 27.12.2013, p. 502-509.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Prevention of H2O2-induced oxidative stress in Chang liver cells by 4-hydroxybenzyl-chitooligomers
AU - Trinh, Mai Duy Luu
AU - Ngo, Dai-Hung
AU - Tran, Dang-Khoa
AU - Tran, Quoc-Tuan
AU - Vo, Thanh-Sang
AU - Dinh, Minh-Hiep
AU - Ngo, Dai-Nghiep
PY - 2013/12/27
Y1 - 2013/12/27
N2 - In this study, a bioactive derivative of chitooligomers (1.0–3.0 kDa), 4-hydroxybenzyl-COS (HB-COS), was synthesized to enhance antioxidant activity. Hence, HB-COS was evaluated for its capabilities against H2O2-induced oxidative stress in human Chang liver cells. It was found that HB-COS possessed the free radical scavenging activity via decreasing the intracellular reactive oxygen species production. Furthermore, HB-COS significantly reduced the oxidation of DNA in a dose-dependent manner. Notably, HB-COS treatment upregulated the gene and protein expressions of antioxidative enzymes and thereby enhancing the intracellular antioxidant mechanisms. In addition, HB-COS treatment caused a remarkable blockade on degradation of inhibitory kappa B alpha (IκB-α) protein and translocation of nuclear factor kappa B (NF-κB). The current study demonstrated that HB-COS effectively attenuated hydrogen peroxide-induced oxidative stress in Chang liver cells by increasing levels of antioxidant enzymes and inhibiting reactive oxygen species generation, DNA oxidation and the NF-κB signaling pathway.
AB - In this study, a bioactive derivative of chitooligomers (1.0–3.0 kDa), 4-hydroxybenzyl-COS (HB-COS), was synthesized to enhance antioxidant activity. Hence, HB-COS was evaluated for its capabilities against H2O2-induced oxidative stress in human Chang liver cells. It was found that HB-COS possessed the free radical scavenging activity via decreasing the intracellular reactive oxygen species production. Furthermore, HB-COS significantly reduced the oxidation of DNA in a dose-dependent manner. Notably, HB-COS treatment upregulated the gene and protein expressions of antioxidative enzymes and thereby enhancing the intracellular antioxidant mechanisms. In addition, HB-COS treatment caused a remarkable blockade on degradation of inhibitory kappa B alpha (IκB-α) protein and translocation of nuclear factor kappa B (NF-κB). The current study demonstrated that HB-COS effectively attenuated hydrogen peroxide-induced oxidative stress in Chang liver cells by increasing levels of antioxidant enzymes and inhibiting reactive oxygen species generation, DNA oxidation and the NF-κB signaling pathway.
KW - Faculty of Science
KW - Chang liver cells
KW - 4-Hydroxybenzyl-chitooligomers (HB-COS)
KW - Free radical scavenging
KW - Antioxidant enzymes
KW - Nuclear factor kappa B (NF-κB)
UR - http://dx.doi.org/10.1016/j.carbpol.2013.12.061
U2 - 10.1016/j.carbpol.2013.12.061
DO - 10.1016/j.carbpol.2013.12.061
M3 - Journal article
VL - 103
SP - 502
EP - 509
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
SN - 0144-8617
ER -
ID: 311345666