A synthetic membrane shaper for controlled liposome deformation
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Dokumenter
- 2021.12.22.473854v1.full
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Shape defines the structure and function of cellular membranes. In cell division, the cell membrane deforms into a ‘dumbbell’ shape, while organelles such as the autophagosome exhibit ‘stomatocyte’ shapes. Bottom-up in vitro reconstitution of protein machineries that stabilize or resolve the membrane necks in such deformed liposome structures is of considerable interest to characterize their function. Here we develop a DNA-nanotechnology-based approach that we call Synthetic Membrane Shaper (SMS), where cholesterol-linked DNA structures attach to the liposome membrane to reproducibly generate high yields of stomatocytes and dumbbells. In silico simulations confirm the shape-stabilizing role of the SMS. We show that the SMS is fully compatible with protein reconstitution by assembling bacterial divisome proteins (DynaminA, FtsZ:ZipA) at the catenoidal neck of these membrane structures. The SMS approach provides a general tool for studying protein binding to complex membrane geometries that will greatly benefit synthetic cell research.
Originalsprog | Engelsk |
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Tidsskrift | bioRxiv |
ISSN | 2692-8205 |
DOI | |
Status | Udgivet - 23 dec. 2021 |
ID: 316411656