Comparative molecular evolution of Trichoderma chitinases in response to mycoparasitic interactions
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Comparative molecular evolution of Trichoderma chitinases in response to mycoparasitic interactions. / Ihrmark, Katarina; Asmail, Nashwan; Ubhayasekera, Wimal; Melin, Petter; Stenlid, Jan; Karlsson, Magnus.
I: Evolutionary Bioinformatics Online, Bind 6, 15.03.2010, s. 1-26.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Comparative molecular evolution of Trichoderma chitinases in response to mycoparasitic interactions
AU - Ihrmark, Katarina
AU - Asmail, Nashwan
AU - Ubhayasekera, Wimal
AU - Melin, Petter
AU - Stenlid, Jan
AU - Karlsson, Magnus
N1 - Keywords: protein evolution, Trichoderma, mycoparasitism, chitinase
PY - 2010/3/15
Y1 - 2010/3/15
N2 - Certain species of the fungal genus Trichoderma are potent mycoparasites and are used for biological control of fungal diseases on agricultural crops. In Trichoderma, whole-genome sequencing reveal between 20 and 36 different genes encoding chitinases, hydrolytic enzymes that are involved in the mycoparasitic attack. Sequences of Trichoderma chitinase genes chi18-5, chi18-13, chi18-15 and chi18-17, which all exhibit specific expression during mycoparasitism-related conditions, were determined from up to 13 different taxa and studied with regard to their evolutionary patterns. Two of them, chi18-13 and chi18-17, are members of the B1/B2 chitinase subgroup that have expanded significantly in paralog number in mycoparasitic Hypocrea atroviridis and H. virens. Chi18-13 contains two codons that evolve under positive selection and seven groups of co-evolving sites. Chi18-15 displays a unique codon-usage and contains five codons that evolve under positive selection and three groups of co-evolving sites. Regions of high amino acid variability are preferentially localized to substrate- or product side of the catalytic clefts. Differences in amino acid diversity/conservation patterns between different Trichoderma clades are observed. These observations show that Trichoderma chitinases chi18-13 and chi18-15 evolve in a manner consistent with rapid co-evolutionary interactions and identifies putative target regions involved in determining substrate-specificity.
AB - Certain species of the fungal genus Trichoderma are potent mycoparasites and are used for biological control of fungal diseases on agricultural crops. In Trichoderma, whole-genome sequencing reveal between 20 and 36 different genes encoding chitinases, hydrolytic enzymes that are involved in the mycoparasitic attack. Sequences of Trichoderma chitinase genes chi18-5, chi18-13, chi18-15 and chi18-17, which all exhibit specific expression during mycoparasitism-related conditions, were determined from up to 13 different taxa and studied with regard to their evolutionary patterns. Two of them, chi18-13 and chi18-17, are members of the B1/B2 chitinase subgroup that have expanded significantly in paralog number in mycoparasitic Hypocrea atroviridis and H. virens. Chi18-13 contains two codons that evolve under positive selection and seven groups of co-evolving sites. Chi18-15 displays a unique codon-usage and contains five codons that evolve under positive selection and three groups of co-evolving sites. Regions of high amino acid variability are preferentially localized to substrate- or product side of the catalytic clefts. Differences in amino acid diversity/conservation patterns between different Trichoderma clades are observed. These observations show that Trichoderma chitinases chi18-13 and chi18-15 evolve in a manner consistent with rapid co-evolutionary interactions and identifies putative target regions involved in determining substrate-specificity.
KW - Former Faculty of Pharmaceutical Sciences
U2 - 10.4137/EBO.S4198
DO - 10.4137/EBO.S4198
M3 - Journal article
C2 - 20454524
VL - 6
SP - 1
EP - 26
JO - Evolutionary Bioinformatics
JF - Evolutionary Bioinformatics
SN - 1176-9343
ER -
ID: 33862610