How Membrane Geometry Regulates Protein Sorting Independently of Mean Curvature
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How Membrane Geometry Regulates Protein Sorting Independently of Mean Curvature. / Larsen, Jannik B.; Rosholm, Kadla R.; Kennard, Celeste; Pedersen, Soren L.; Munch, Henrik K.; Tkach, Vadym; Sakon, John J.; Bjornholm, Thomas; Weninger, Keith R.; Bendix, Poul Martin; Jensen, Knud J.; Hatzakis, Nikos S.; Uline, Mark J.; Stamou, Dimitrios.
I: ACS Central Science, Bind 6, Nr. 7, 2020, s. 1159-1168.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - How Membrane Geometry Regulates Protein Sorting Independently of Mean Curvature
AU - Larsen, Jannik B.
AU - Rosholm, Kadla R.
AU - Kennard, Celeste
AU - Pedersen, Soren L.
AU - Munch, Henrik K.
AU - Tkach, Vadym
AU - Sakon, John J.
AU - Bjornholm, Thomas
AU - Weninger, Keith R.
AU - Bendix, Poul Martin
AU - Jensen, Knud J.
AU - Hatzakis, Nikos S.
AU - Uline, Mark J.
AU - Stamou, Dimitrios
PY - 2020
Y1 - 2020
N2 - Biological membranes have distinct geometries that confer specific functions. However, the molecular mechanisms underlying the phenomenological geometry/function correlations remain elusive. We studied the effect of membrane geometry on the localization of membrane-bound proteins. Quantitative comparative experiments between the two most abundant cellular membrane geometries, spherical and cylindrical, revealed that geometry regulates the spatial segregation of proteins. The measured geometry-driven segregation reached 50-fold for membranes of the same mean curvature, demonstrating a crucial and hitherto unaccounted contribution by Gaussian curvature. Molecular-field theory calculations elucidated the underlying physical and molecular mechanisms. Our results reveal that distinct membrane geometries have specific physicochemical properties and thus establish a ubiquitous mechanistic foundation for unravelling the conserved correlations between biological function and membrane polymorphism.
AB - Biological membranes have distinct geometries that confer specific functions. However, the molecular mechanisms underlying the phenomenological geometry/function correlations remain elusive. We studied the effect of membrane geometry on the localization of membrane-bound proteins. Quantitative comparative experiments between the two most abundant cellular membrane geometries, spherical and cylindrical, revealed that geometry regulates the spatial segregation of proteins. The measured geometry-driven segregation reached 50-fold for membranes of the same mean curvature, demonstrating a crucial and hitherto unaccounted contribution by Gaussian curvature. Molecular-field theory calculations elucidated the underlying physical and molecular mechanisms. Our results reveal that distinct membrane geometries have specific physicochemical properties and thus establish a ubiquitous mechanistic foundation for unravelling the conserved correlations between biological function and membrane polymorphism.
KW - AMPHIPATHIC HELICES
KW - SYNAPTOTAGMIN
KW - MECHANISMS
KW - LOCALIZATION
KW - AMPHIPHYSIN
KW - INDUCE
KW - DOMAIN
KW - CELL
U2 - 10.1021/acscentsci.0c00419
DO - 10.1021/acscentsci.0c00419
M3 - Journal article
C2 - 32724850
VL - 6
SP - 1159
EP - 1168
JO - A C S Central Science
JF - A C S Central Science
SN - 2374-7943
IS - 7
ER -
ID: 247155911