Optical investigation of intra- and extra-cellular dynamics
Publikation: Bog/antologi/afhandling/rapport › Ph.d.-afhandling › Forskning
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Optical investigation of intra- and extra-cellular dynamics. / Leijnse, Natascha.
The Niels Bohr Institute, Faculty of Science, University of Copenhagen, 2013. 132 s.Publikation: Bog/antologi/afhandling/rapport › Ph.d.-afhandling › Forskning
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TY - BOOK
T1 - Optical investigation of intra- and extra-cellular dynamics
AU - Leijnse, Natascha
N1 - Thesis is not public.
PY - 2013
Y1 - 2013
N2 - In this thesis we investigated intra- and extra-cellular dynamics with the help of optical tweezers and confocal imaging.Firstly, we measured in vivo diffusion inside endothelial cells by tracking lipid granules and compared the results to in vitro diffusion of polystyrene beads in wormlike micelles.Furthermore, we studied F-actin dynamics in, and curvature sensing of, a receptor and its ligand on artificially pulled membrane tethers.Force measurements between a cell and an approaching optically trapped bead were conducted in order to probe the endothelial cell's glycocalyx.Finally, investigations of force generation by living cells embedded in 3D matrices, conducted in Alexander Dunn's lab at Stanford University, are described in the last chapter.
AB - In this thesis we investigated intra- and extra-cellular dynamics with the help of optical tweezers and confocal imaging.Firstly, we measured in vivo diffusion inside endothelial cells by tracking lipid granules and compared the results to in vitro diffusion of polystyrene beads in wormlike micelles.Furthermore, we studied F-actin dynamics in, and curvature sensing of, a receptor and its ligand on artificially pulled membrane tethers.Force measurements between a cell and an approaching optically trapped bead were conducted in order to probe the endothelial cell's glycocalyx.Finally, investigations of force generation by living cells embedded in 3D matrices, conducted in Alexander Dunn's lab at Stanford University, are described in the last chapter.
UR - https://soeg.kb.dk/permalink/45KBDK_KGL/fbp0ps/alma99122889237105763
M3 - Ph.D. thesis
BT - Optical investigation of intra- and extra-cellular dynamics
PB - The Niels Bohr Institute, Faculty of Science, University of Copenhagen
ER -
ID: 88025319