Synthetic Membrane Shaper for Controlled Liposome Deformation
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Dokumenter
- acsnano.2c06125
Forlagets udgivne version, 10,7 MB, PDF-dokument
Shape defines the structure and function of cellular membranes. In cell division, the cell membrane deforms into a "dumbbell" shape, while organelles such as the autophagosome exhibit "stomatocyte" shapes. Bottom-up in vitro reconstitution of protein machineries that stabilize or resolve the membrane necks in such deformed liposome structures is of considerable interest to characterize their function. Here we develop a DNA-nanotechnology-based approach that we call the synthetic membrane shaper (SMS), where cholesterol-linked DNA structures attach to the liposome membrane to reproducibly generate high yields of stomatocytes and dumbbells. In silico simulations confirm the shape-stabilizing role of the SMS. We show that the SMS is fully compatible with protein reconstitution by assembling bacterial divisome proteins (DynaminA, FtsZ:ZipA) at the catenoidal neck of these membrane structures. The SMS approach provides a general tool for studying protein binding to complex membrane geometries that will greatly benefit synthetic cell research.
Originalsprog | Engelsk |
---|---|
Tidsskrift | ACS Nano |
Vol/bind | 17 |
Sider (fra-til) | 966-978 |
Antal sider | 13 |
ISSN | 1936-0851 |
DOI | |
Status | Udgivet - 28 nov. 2022 |
ID: 328042263