Towards in vivo applications of111 Ag perturbed angular correlation of γ-rays (PAC) spectroscopy
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Towards in vivo applications of111 Ag perturbed angular correlation of γ-rays (PAC) spectroscopy. / Tosato, Marianna; Asti, Mattia; Di Marco, Valerio; Jensen, Marianne L.; Schell, Juliana; Dang, Thien Thanh; Koster, Ulli; Jensen, Mikael; Hemmingsen, Lars.
I: Applied Radiation and Isotopes, Bind 190, 110508, 2022.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Towards in vivo applications of111 Ag perturbed angular correlation of γ-rays (PAC) spectroscopy
AU - Tosato, Marianna
AU - Asti, Mattia
AU - Di Marco, Valerio
AU - Jensen, Marianne L.
AU - Schell, Juliana
AU - Dang, Thien Thanh
AU - Koster, Ulli
AU - Jensen, Mikael
AU - Hemmingsen, Lars
PY - 2022
Y1 - 2022
N2 - 111Ag-perturbed angular correlation of gamma-rays (PAC) spectroscopy provides information on the nuclear quadrupole interactions, and thereby on the local structure and dynamics of the silver ion binding site. Brownian rotational motion, i.e. rotational diffusion, of 111Ag-labeled molecules will significantly affect the PAC spectra. Here we illustrate this effect, by simulating 111Ag PAC spectra for 111Ag-labeled molecules with molecular masses spanning from 102 to 106 g/mol, reflecting a span from fast (small molecules) to slow (large molecules) rotational diffusion on the PAC time scale. The simulated spectra are compared to 111Ag-PAC data obtained from a pilot study involving 111Ag(I) bound to a designed chelator exhibiting fast reorientation in solution, as well as to 111Ag-labeled species formed by 111Ag(I) in human serum, exhibiting slow (or no) reorientation on the PAC time scale. The simulated and experimental data illustrate typical PAC signals that are likely to be observed in vivo, when following the fate of 111Ag-labeled compounds. Potential in vivo applications are stability studies of 111Ag-radiopharmaceuticals, dissociation studies of 111Ag from the labeled molecule followed by binding to another (bio)molecule, or binding of 111Ag-labeled probes to larger carriers such as proteins.
AB - 111Ag-perturbed angular correlation of gamma-rays (PAC) spectroscopy provides information on the nuclear quadrupole interactions, and thereby on the local structure and dynamics of the silver ion binding site. Brownian rotational motion, i.e. rotational diffusion, of 111Ag-labeled molecules will significantly affect the PAC spectra. Here we illustrate this effect, by simulating 111Ag PAC spectra for 111Ag-labeled molecules with molecular masses spanning from 102 to 106 g/mol, reflecting a span from fast (small molecules) to slow (large molecules) rotational diffusion on the PAC time scale. The simulated spectra are compared to 111Ag-PAC data obtained from a pilot study involving 111Ag(I) bound to a designed chelator exhibiting fast reorientation in solution, as well as to 111Ag-labeled species formed by 111Ag(I) in human serum, exhibiting slow (or no) reorientation on the PAC time scale. The simulated and experimental data illustrate typical PAC signals that are likely to be observed in vivo, when following the fate of 111Ag-labeled compounds. Potential in vivo applications are stability studies of 111Ag-radiopharmaceuticals, dissociation studies of 111Ag from the labeled molecule followed by binding to another (bio)molecule, or binding of 111Ag-labeled probes to larger carriers such as proteins.
KW - COORDINATION GEOMETRY
KW - LIPID VESICLES
KW - METAL-IONS
KW - STABILITY
KW - STATE
KW - RADIOMETALS
KW - LIPOSOMES
KW - SILVER(I)
KW - COMPLEX
KW - BINDING
U2 - 10.1016/j.apradiso.2022.110508
DO - 10.1016/j.apradiso.2022.110508
M3 - Journal article
C2 - 36283328
VL - 190
JO - Applied Radiation and Isotopes
JF - Applied Radiation and Isotopes
SN - 0969-8043
M1 - 110508
ER -
ID: 327056137