Skin biomarkers predict development of atopic dermatitis in infancy
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Skin biomarkers predict development of atopic dermatitis in infancy. / Rinnov, Maria Rasmussen; Halling, Anne-Sofie; Gerner, Trine; Ravn, Nina Haarup; Knudgaard, Mette Hjorslev; Trautner, Simon; Goorden, Susan M.; Ghauharali-van der Vlugt, Karen J. M.; Stet, Femke S.; Skov, Lone; Thomsen, Simon Francis; Egeberg, Alexander; Rosted, Aske L. L.; Petersen, Troels; Jakasa, Ivone; Riethmuller, Christoph; Kezic, Sanja; Thyssen, Jacob P.
In: Allergy, Vol. 78, No. 3, 2023, p. 791-802.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Skin biomarkers predict development of atopic dermatitis in infancy
AU - Rinnov, Maria Rasmussen
AU - Halling, Anne-Sofie
AU - Gerner, Trine
AU - Ravn, Nina Haarup
AU - Knudgaard, Mette Hjorslev
AU - Trautner, Simon
AU - Goorden, Susan M.
AU - Ghauharali-van der Vlugt, Karen J. M.
AU - Stet, Femke S.
AU - Skov, Lone
AU - Thomsen, Simon Francis
AU - Egeberg, Alexander
AU - Rosted, Aske L. L.
AU - Petersen, Troels
AU - Jakasa, Ivone
AU - Riethmuller, Christoph
AU - Kezic, Sanja
AU - Thyssen, Jacob P.
PY - 2023
Y1 - 2023
N2 - Background There is currently no insight into biomarkers that can predict the onset of pediatric atopic dermatitis (AD). Methods Nested in a prospective birth cohort study that examined the occurrence of physician-diagnosed AD in 300 children, 44 random children with onset of AD in the first year of life were matched on sex and season of birth with 44 children who did not develop AD. Natural moisturizing factor (NMF), corneocyte surface protrusions, cytokines, free sphingoid bases (SBs) of different chain lengths and their ceramides were analyzed from tape strips collected at 2 months of age before onset of AD using liquid chromatography, atomic force microscopy, multiplex immunoassay, and liquid chromatography mass spectrometry, respectively. Results Significant alterations were observed for four lipid markers, with phytosphingosine ([P]) levels being significantly lower in children who developed AD compared with children who did not (median 240 pmol/mg vs. 540 pmol/mg, p < 0.001). The two groups of children differed in the relative amounts of SB of different chain lengths (C17, C18 and C20). Thymus- and activation-regulated chemokine (TARC/CCL17) was slightly higher in children who developed AD, whereas NMF and corneocyte surface texture were similar. AD severity assessed by the eczema area and severity index (EASI) at disease onset was 4.2 (2.0;7.2). [P] had the highest prediction accuracy among the biomarkers (75.6%), whereas the combination of 5 lipid ratios gave an accuracy of 89.4%. Conclusion This study showed that levels and SB chain length were altered in infants who later developed AD, and that TARC/CCL17 levels were higher.
AB - Background There is currently no insight into biomarkers that can predict the onset of pediatric atopic dermatitis (AD). Methods Nested in a prospective birth cohort study that examined the occurrence of physician-diagnosed AD in 300 children, 44 random children with onset of AD in the first year of life were matched on sex and season of birth with 44 children who did not develop AD. Natural moisturizing factor (NMF), corneocyte surface protrusions, cytokines, free sphingoid bases (SBs) of different chain lengths and their ceramides were analyzed from tape strips collected at 2 months of age before onset of AD using liquid chromatography, atomic force microscopy, multiplex immunoassay, and liquid chromatography mass spectrometry, respectively. Results Significant alterations were observed for four lipid markers, with phytosphingosine ([P]) levels being significantly lower in children who developed AD compared with children who did not (median 240 pmol/mg vs. 540 pmol/mg, p < 0.001). The two groups of children differed in the relative amounts of SB of different chain lengths (C17, C18 and C20). Thymus- and activation-regulated chemokine (TARC/CCL17) was slightly higher in children who developed AD, whereas NMF and corneocyte surface texture were similar. AD severity assessed by the eczema area and severity index (EASI) at disease onset was 4.2 (2.0;7.2). [P] had the highest prediction accuracy among the biomarkers (75.6%), whereas the combination of 5 lipid ratios gave an accuracy of 89.4%. Conclusion This study showed that levels and SB chain length were altered in infants who later developed AD, and that TARC/CCL17 levels were higher.
KW - atopic dermatitis
KW - biomarker
KW - birth cohort
KW - prediction
KW - skin barrier
KW - STRATUM-CORNEUM
KW - SERINE PALMITOYLTRANSFERASE
KW - BARRIER FUNCTION
KW - SPHINGOID BASES
KW - FILAGGRIN
KW - ECZEMA
KW - PHYTOSPHINGOSINE
KW - LEVEL
KW - QUANTIFICATION
KW - DEGRADATION
U2 - 10.1111/all.15518
DO - 10.1111/all.15518
M3 - Journal article
C2 - 36112082
VL - 78
SP - 791
EP - 802
JO - Allergy: European Journal of Allergy and Clinical Immunology
JF - Allergy: European Journal of Allergy and Clinical Immunology
SN - 0105-4538
IS - 3
ER -
ID: 322114557