Synthetic Membrane Shaper for Controlled Liposome Deformation
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Synthetic Membrane Shaper for Controlled Liposome Deformation. / De Franceschi, Nicola; Pezeshkian, Weria; Fragasso, Alessio; Bruininks, Bart M H; Tsai, Sean; Marrink, Siewert J; Dekker, Cees.
In: ACS Nano, Vol. 17, 28.11.2022, p. 966-978.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Synthetic Membrane Shaper for Controlled Liposome Deformation
AU - De Franceschi, Nicola
AU - Pezeshkian, Weria
AU - Fragasso, Alessio
AU - Bruininks, Bart M H
AU - Tsai, Sean
AU - Marrink, Siewert J
AU - Dekker, Cees
PY - 2022/11/28
Y1 - 2022/11/28
N2 - Shape defines the structure and function of cellular membranes. In cell division, the cell membrane deforms into a "dumbbell" shape, while organelles such as the autophagosome exhibit "stomatocyte" shapes. Bottom-up in vitro reconstitution of protein machineries that stabilize or resolve the membrane necks in such deformed liposome structures is of considerable interest to characterize their function. Here we develop a DNA-nanotechnology-based approach that we call the synthetic membrane shaper (SMS), where cholesterol-linked DNA structures attach to the liposome membrane to reproducibly generate high yields of stomatocytes and dumbbells. In silico simulations confirm the shape-stabilizing role of the SMS. We show that the SMS is fully compatible with protein reconstitution by assembling bacterial divisome proteins (DynaminA, FtsZ:ZipA) at the catenoidal neck of these membrane structures. The SMS approach provides a general tool for studying protein binding to complex membrane geometries that will greatly benefit synthetic cell research.
AB - Shape defines the structure and function of cellular membranes. In cell division, the cell membrane deforms into a "dumbbell" shape, while organelles such as the autophagosome exhibit "stomatocyte" shapes. Bottom-up in vitro reconstitution of protein machineries that stabilize or resolve the membrane necks in such deformed liposome structures is of considerable interest to characterize their function. Here we develop a DNA-nanotechnology-based approach that we call the synthetic membrane shaper (SMS), where cholesterol-linked DNA structures attach to the liposome membrane to reproducibly generate high yields of stomatocytes and dumbbells. In silico simulations confirm the shape-stabilizing role of the SMS. We show that the SMS is fully compatible with protein reconstitution by assembling bacterial divisome proteins (DynaminA, FtsZ:ZipA) at the catenoidal neck of these membrane structures. The SMS approach provides a general tool for studying protein binding to complex membrane geometries that will greatly benefit synthetic cell research.
U2 - 10.1021/acsnano.2c06125
DO - 10.1021/acsnano.2c06125
M3 - Journal article
C2 - 36441529
VL - 17
SP - 966
EP - 978
JO - A C S Nano
JF - A C S Nano
SN - 1936-0851
ER -
ID: 328042263