The insulin-sensitizing effect of a single exercise bout is similar in type I and type II human muscle fibres
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The insulin-sensitizing effect of a single exercise bout is similar in type I and type II human muscle fibres. / Larsen, Magnus Romme; Steenberg, Dorte Enggaard; Birk, Jesper Bratz; Sjøberg, Kim Anker; Kiens, Bente; Richter, Erik A.; Wojtaszewski, Jørgen.
In: Journal of Physiology, Vol. 598, No. 24, 2020, p. 5687-5699.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - The insulin-sensitizing effect of a single exercise bout is similar in type I and type II human muscle fibres
AU - Larsen, Magnus Romme
AU - Steenberg, Dorte Enggaard
AU - Birk, Jesper Bratz
AU - Sjøberg, Kim Anker
AU - Kiens, Bente
AU - Richter, Erik A.
AU - Wojtaszewski, Jørgen
N1 - CURIS 2020 NEXS 323
PY - 2020
Y1 - 2020
N2 - Human skeletal muscle consists of slow-twitch (type I) and fast-twitch (type II) muscle fibres. Muscle insulin action, regulating glucose uptake and metabolism, is improved following a single exercise bout. Rodent studies suggest that this phenomenon is confined to specific muscle fibre types. Whether this phenomenon is also confined to specific fibre types in humans has not been described. To investigate this, nine healthy men underwent a euglycemic hyperinsulinemic clamp (EHC) in the recovery from a single bout of one-legged knee-extensor exercise. Pools of type I and type II fibres were prepared from muscle biopsies taken in the rested and prior exercised leg before and after theEHC. AMPK γ3 and TBC1D4 – two key proteins regulating muscle insulin action following exercise – were higher expressed in type II compared to type I fibres. However, phosphor-regulation of TBC1D4 was similar between fibre types when related to the total amount of TBC1D4 protein. The activating dephosphorylation of glycogen synthase was also similar in the two fibre types. Thus, insulin-induced regulation of key proteins important for transport and intracellular flux of glucose towards glycogen storage in the recovery from exercise, does not differ between fibre types. In conclusion, the insulinsensitizing effect of a single bout of exercise includes both type I and type II fibres in human skeletal muscle. This may be an important observation for future pharmacological strategies targeting muscle insulin sensitivity in humans.
AB - Human skeletal muscle consists of slow-twitch (type I) and fast-twitch (type II) muscle fibres. Muscle insulin action, regulating glucose uptake and metabolism, is improved following a single exercise bout. Rodent studies suggest that this phenomenon is confined to specific muscle fibre types. Whether this phenomenon is also confined to specific fibre types in humans has not been described. To investigate this, nine healthy men underwent a euglycemic hyperinsulinemic clamp (EHC) in the recovery from a single bout of one-legged knee-extensor exercise. Pools of type I and type II fibres were prepared from muscle biopsies taken in the rested and prior exercised leg before and after theEHC. AMPK γ3 and TBC1D4 – two key proteins regulating muscle insulin action following exercise – were higher expressed in type II compared to type I fibres. However, phosphor-regulation of TBC1D4 was similar between fibre types when related to the total amount of TBC1D4 protein. The activating dephosphorylation of glycogen synthase was also similar in the two fibre types. Thus, insulin-induced regulation of key proteins important for transport and intracellular flux of glucose towards glycogen storage in the recovery from exercise, does not differ between fibre types. In conclusion, the insulinsensitizing effect of a single bout of exercise includes both type I and type II fibres in human skeletal muscle. This may be an important observation for future pharmacological strategies targeting muscle insulin sensitivity in humans.
KW - Faculty of Science
KW - Glucose metabolism
KW - Insulin sensitivity
KW - Insulin signalling
KW - Muscle fibre type
U2 - 10.1113/JP280475
DO - 10.1113/JP280475
M3 - Journal article
C2 - 32916040
VL - 598
SP - 5687
EP - 5699
JO - The Journal of Physiology
JF - The Journal of Physiology
SN - 0022-3751
IS - 24
ER -
ID: 248547600