Label-free optical interferometric microscopy to characterize morphodynamics in living plants

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During the last century, fluorescence microscopy has played a pivotal role in a range of scientific discoveries. The success of fluorescence microscopy has prevailed despite several shortcomings like measurement time, photobleaching, temporal resolution, and specific sample preparation. To bypass these obstacles, label-free interferometric methods have been developed. Interferometry exploits the full wavefront information of laser light after interaction with biological material to yield interference patterns that contain information about structure and activity. Here, we review recent studies in interferometric imaging of plant cells and tissues, using techniques such as biospeckle imaging, optical coherence tomography, and digital holography. These methods enable quantification of cell morphology and dynamic intracellular measurements over extended periods of time. Recent investigations have showcased the potential of interferometric techniques for precise identification of seed viability and germination, plant diseases, plant growth and cell texture, intracellular activity and cytoplasmic transport. We envision that further developments of these label-free approaches, will allow for high-resolution, dynamic imaging of plants and their organelles, ranging in scales from sub-cellular to tissue and from milliseconds to hours.

Original languageEnglish
Article number1156478
JournalFrontiers in Plant Science
Number of pages8
Publication statusPublished - 2023

    Research areas

  • digital holographic cell imaging, optical coherence tomography, label-free microscopy, material transport, intereferometric imaging, plant cells and tissues, speckle imaging, plant morphodynamics, DIGITAL HOLOGRAPHIC MICROSCOPY, COHERENCE TOMOGRAPHY, BIOSPECKLE ACTIVITY, ROOT-GROWTH, REVEALS, DIFFRACTION, DYNAMICS, LEAF, TOOL

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