Multiciliated cells use filopodia to probe tissue mechanics during epithelial integration in vivo

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Multiciliated cells use filopodia to probe tissue mechanics during epithelial integration in vivo. / Ventura, Guilherme; Amiri, Aboutaleb; Thiagarajan, Raghavan; Tolonen, Mari; Doostmohammadi, Amin; Sedzinski, Jakub.

In: Nature Communications, Vol. 13, No. 1, 6423, 2022.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Ventura, G, Amiri, A, Thiagarajan, R, Tolonen, M, Doostmohammadi, A & Sedzinski, J 2022, 'Multiciliated cells use filopodia to probe tissue mechanics during epithelial integration in vivo', Nature Communications, vol. 13, no. 1, 6423. https://doi.org/10.1038/s41467-022-34165-0

APA

Ventura, G., Amiri, A., Thiagarajan, R., Tolonen, M., Doostmohammadi, A., & Sedzinski, J. (2022). Multiciliated cells use filopodia to probe tissue mechanics during epithelial integration in vivo. Nature Communications, 13(1), [6423]. https://doi.org/10.1038/s41467-022-34165-0

Vancouver

Ventura G, Amiri A, Thiagarajan R, Tolonen M, Doostmohammadi A, Sedzinski J. Multiciliated cells use filopodia to probe tissue mechanics during epithelial integration in vivo. Nature Communications. 2022;13(1). 6423. https://doi.org/10.1038/s41467-022-34165-0

Author

Ventura, Guilherme ; Amiri, Aboutaleb ; Thiagarajan, Raghavan ; Tolonen, Mari ; Doostmohammadi, Amin ; Sedzinski, Jakub. / Multiciliated cells use filopodia to probe tissue mechanics during epithelial integration in vivo. In: Nature Communications. 2022 ; Vol. 13, No. 1.

Bibtex

@article{5e965859cf9a4f1ab50cb47d207b82a1,

title = "Multiciliated cells use filopodia to probe tissue mechanics during epithelial integration in vivo",

abstract = "During embryonic development, regeneration, and homeostasis, cells have to migrate and physically integrate into the target tissues where they ultimately execute their function. While much is known about the biochemical pathways driving cell migration in vivo, we are only beginning to understand the mechanical interplay between migrating cells and their surrounding tissue. Here, we reveal that multiciliated cell precursors in the Xenopus embryo use filopodia to pull at the vertices of the overlying epithelial sheet. This pulling is effectively used to sense vertex stiffness and identify the preferred positions for cell integration into the tissue. Notably, we find that pulling forces equip multiciliated cells with the ability to remodel the epithelial junctions of the neighboring cells, enabling them to generate a permissive environment that facilitates integration. Our findings reveal the intricate physical crosstalk at the cell-tissue interface and uncover previously unknown functions for mechanical forces in orchestrating cell integration.",

author = "Guilherme Ventura and Aboutaleb Amiri and Raghavan Thiagarajan and Mari Tolonen and Amin Doostmohammadi and Jakub Sedzinski",

note = "Publisher Copyright: {\textcopyright} 2022, The Author(s).",

year = "2022",

doi = "10.1038/s41467-022-34165-0",

language = "English",

volume = "13",

journal = "Nature Communications",

issn = "2041-1723",

publisher = "nature publishing group",

number = "1",

}

RIS

TY - JOUR

T1 - Multiciliated cells use filopodia to probe tissue mechanics during epithelial integration in vivo

AU - Ventura, Guilherme

AU - Amiri, Aboutaleb

AU - Thiagarajan, Raghavan

AU - Tolonen, Mari

AU - Doostmohammadi, Amin

AU - Sedzinski, Jakub

PY - 2022

Y1 - 2022

N2 - During embryonic development, regeneration, and homeostasis, cells have to migrate and physically integrate into the target tissues where they ultimately execute their function. While much is known about the biochemical pathways driving cell migration in vivo, we are only beginning to understand the mechanical interplay between migrating cells and their surrounding tissue. Here, we reveal that multiciliated cell precursors in the Xenopus embryo use filopodia to pull at the vertices of the overlying epithelial sheet. This pulling is effectively used to sense vertex stiffness and identify the preferred positions for cell integration into the tissue. Notably, we find that pulling forces equip multiciliated cells with the ability to remodel the epithelial junctions of the neighboring cells, enabling them to generate a permissive environment that facilitates integration. Our findings reveal the intricate physical crosstalk at the cell-tissue interface and uncover previously unknown functions for mechanical forces in orchestrating cell integration.

AB - During embryonic development, regeneration, and homeostasis, cells have to migrate and physically integrate into the target tissues where they ultimately execute their function. While much is known about the biochemical pathways driving cell migration in vivo, we are only beginning to understand the mechanical interplay between migrating cells and their surrounding tissue. Here, we reveal that multiciliated cell precursors in the Xenopus embryo use filopodia to pull at the vertices of the overlying epithelial sheet. This pulling is effectively used to sense vertex stiffness and identify the preferred positions for cell integration into the tissue. Notably, we find that pulling forces equip multiciliated cells with the ability to remodel the epithelial junctions of the neighboring cells, enabling them to generate a permissive environment that facilitates integration. Our findings reveal the intricate physical crosstalk at the cell-tissue interface and uncover previously unknown functions for mechanical forces in orchestrating cell integration.

U2 - 10.1038/s41467-022-34165-0

DO - 10.1038/s41467-022-34165-0

M3 - Journal article

C2 - 36307428

AN - SCOPUS:85140787858

VL - 13

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

IS - 1

M1 - 6423

ER -

ID: 325027444

Niels Bohr Institute