New methods and reagents to improve the ferret model for human influenza infections
Publikation: Konferencebidrag › Poster › Forskning
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New methods and reagents to improve the ferret model for human influenza infections. / Martel, Cyril Jean-Marie; Kirkeby, Svend; Aasted, Bent.
2008. Poster session præsenteret ved Graduate School of Immunology 3. Annual Meeting: Interphases between adaptive and innate immunity in inflammation and autoimmunity, Helsingør, Danmark.Publikation: Konferencebidrag › Poster › Forskning
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TY - CONF
T1 - New methods and reagents to improve the ferret model for human influenza infections
AU - Martel, Cyril Jean-Marie
AU - Kirkeby, Svend
AU - Aasted, Bent
N1 - Conference code: 3
PY - 2008
Y1 - 2008
N2 - The ferret has been extensively used to study human influenza infections. However, its value as a model has suffered from the limited set of reagents and methods available for this animal. We have recently tested a large number of monoclonal antibodies cross-reacting with ferret CD markers (CD8, CD9, CD14, CD18, CD25, CD29, CD32, CD44, CD61, CD71, CD79b, CD88, CD104, CD172a and CD3) and cytokines (interferon-gamma, TNF-alpha, interleukine-4 and interleukine-8) for flow cytometry , as well as polyclonal antibodies cross-reacting with ferret immunoglobulins (IgA, IgG and IgM) for ELISA. Further improvements of the model will aim at establishing a reliable RT-PCR for ferret cytokines, as well as investigating the location of influenza receptors and viral particles in the upper and lower respiratory tract via immunohistochemistry
AB - The ferret has been extensively used to study human influenza infections. However, its value as a model has suffered from the limited set of reagents and methods available for this animal. We have recently tested a large number of monoclonal antibodies cross-reacting with ferret CD markers (CD8, CD9, CD14, CD18, CD25, CD29, CD32, CD44, CD61, CD71, CD79b, CD88, CD104, CD172a and CD3) and cytokines (interferon-gamma, TNF-alpha, interleukine-4 and interleukine-8) for flow cytometry , as well as polyclonal antibodies cross-reacting with ferret immunoglobulins (IgA, IgG and IgM) for ELISA. Further improvements of the model will aim at establishing a reliable RT-PCR for ferret cytokines, as well as investigating the location of influenza receptors and viral particles in the upper and lower respiratory tract via immunohistochemistry
KW - Former LIFE faculty
KW - ferret
KW - monoclonal antibodies
KW - CD markers
KW - cytokines
KW - immunoglobulins
M3 - Poster
Y2 - 1 October 2008 through 3 October 2008
ER -
ID: 8115842