Removal of damaged proteins during ES cell fate specification requires the proteasome activator PA28
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Standard
Removal of damaged proteins during ES cell fate specification requires the proteasome activator PA28. / Hernebring, Malin; Fredriksson, Asa; Liljevald, Maria; Cvijovic, Marija; Norrman, Karin; Wiseman, John; Semb, Tor Henrik; Nyström, Thomas.
I: Scientific Reports, Bind 3, 05.03.2013, s. 1381.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Removal of damaged proteins during ES cell fate specification requires the proteasome activator PA28
AU - Hernebring, Malin
AU - Fredriksson, Asa
AU - Liljevald, Maria
AU - Cvijovic, Marija
AU - Norrman, Karin
AU - Wiseman, John
AU - Semb, Tor Henrik
AU - Nyström, Thomas
PY - 2013/3/5
Y1 - 2013/3/5
N2 - In embryonic stem cells, removal of oxidatively damaged proteins is triggered upon the first signs of cell fate specification but the underlying mechanism is not known. Here, we report that this phase of differentiation encompasses an unexpected induction of genes encoding the proteasome activator PA28aß (11S), subunits of the immunoproteasome (20Si), and the 20Si regulator TNFa. This induction is accompanied by assembly of mature PA28-20S(i) proteasomes and elevated proteasome activity. Inhibiting accumulation of PA28a using miRNA counteracted the removal of damaged proteins demonstrating that PA28aß has a hitherto unidentified role required for resetting the levels of protein damage at the transition from self-renewal to cell differentiation.
AB - In embryonic stem cells, removal of oxidatively damaged proteins is triggered upon the first signs of cell fate specification but the underlying mechanism is not known. Here, we report that this phase of differentiation encompasses an unexpected induction of genes encoding the proteasome activator PA28aß (11S), subunits of the immunoproteasome (20Si), and the 20Si regulator TNFa. This induction is accompanied by assembly of mature PA28-20S(i) proteasomes and elevated proteasome activity. Inhibiting accumulation of PA28a using miRNA counteracted the removal of damaged proteins demonstrating that PA28aß has a hitherto unidentified role required for resetting the levels of protein damage at the transition from self-renewal to cell differentiation.
KW - Faculty of Health and Medical Sciences
KW - Embryonic Stem Cells
KW - PROTEIN QUALITY control
KW - STEM-CELL DIFFERENTIATION
KW - PROTEASOME
U2 - 10.1038/srep01381
DO - 10.1038/srep01381
M3 - Journal article
C2 - 23459332
VL - 3
SP - 1381
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
ER -
ID: 44739796