Genetic selection and DNA sequences of 4.5S RNA homologs
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Genetic selection and DNA sequences of 4.5S RNA homologs. / Brown, S; Thon, G; Tolentino, E.
I: Journal of Bacteriology, Bind 171, Nr. 12, 1989, s. 6517-20.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Genetic selection and DNA sequences of 4.5S RNA homologs
AU - Brown, S
AU - Thon, G
AU - Tolentino, E
N1 - Keywords: Bacillus subtilis; Bacteria; Base Sequence; Cloning, Molecular; DNA, Ribosomal; Escherichia coli; Genes, Bacterial; Genetic Complementation Test; Genomic Library; Molecular Sequence Data; Nucleic Acid Conformation; RNA, Ribosomal; Selection (Genetics); Sequence Homology, Nucleic Acid; Species Specificity
PY - 1989
Y1 - 1989
N2 - A general strategy for cloning the functional homologs of an Escherichia coli gene was used to clone homologs of 4.5S RNA from other bacteria. The genes encoding these homologs were selected by their ability to complement a deletion of the gene for 4.5S RNA. DNA sequences of the regions encoding the homologs were determined. Since this approach does not require that the homologous genes hybridize with probes generated from the E. coli sequence, the sequences of the homologs were not all similar to the sequence of the E. coli gene. Despite the dissimilarity of the primary sequences of some of the homologs, all could be folded to obtain a similar structure.
AB - A general strategy for cloning the functional homologs of an Escherichia coli gene was used to clone homologs of 4.5S RNA from other bacteria. The genes encoding these homologs were selected by their ability to complement a deletion of the gene for 4.5S RNA. DNA sequences of the regions encoding the homologs were determined. Since this approach does not require that the homologous genes hybridize with probes generated from the E. coli sequence, the sequences of the homologs were not all similar to the sequence of the E. coli gene. Despite the dissimilarity of the primary sequences of some of the homologs, all could be folded to obtain a similar structure.
M3 - Journal article
C2 - 2512280
VL - 171
SP - 6517
EP - 6520
JO - Journal of Bacteriology
JF - Journal of Bacteriology
SN - 0021-9193
IS - 12
ER -
ID: 9297184