Membrane Tubulation in Lipid Vesicles Triggered by the Local Application of Calcium Ions
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Standard
Membrane Tubulation in Lipid Vesicles Triggered by the Local Application of Calcium Ions. / Doosti, Baharan Ali; Pezeshkian, Weria; Bruhn, Dennis S.; Ipsen, John H.; Khandelia, Himanshu; Jeffries, Gavin D. M.; Lobovidna, Tatsiana.
I: Langmuir, Bind 33, Nr. 41, 17.10.2017, s. 11010-11017.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Membrane Tubulation in Lipid Vesicles Triggered by the Local Application of Calcium Ions
AU - Doosti, Baharan Ali
AU - Pezeshkian, Weria
AU - Bruhn, Dennis S.
AU - Ipsen, John H.
AU - Khandelia, Himanshu
AU - Jeffries, Gavin D. M.
AU - Lobovidna, Tatsiana
PY - 2017/10/17
Y1 - 2017/10/17
N2 - Experimental and theoretical studies on ion-lipid interactions predict that binding of calcium ions to cell membranes leads to macroscopic mechanical effects and membrane remodeling. Herein, we provide experimental evidence that a point source of Ca2+ acting upon a negatively charged membrane generates spontaneous curvature and triggers the formation of tubular protrusions that point away from the ion source. This behavior is rationalized by strong binding of the divalent cations to the surface of the charged bilayer, which effectively neutralizes the surface charge density of outer leaflet of the bilayer. The mismatch in the surface charge density of the two leaflets leads to nonzero spontaneous curvature. We probe this mismatch through the use of molecular dynamics simulations and validate that calcium ion binding to a lipid membrane is sufficient to generate inward spontaneous curvature, bending the membrane. Additionally, we demonstrate that the formed tubular protrusions can be translated along the vesicle surface in a controlled manner by repositioning the site of localized Ca2+ exposure. The findings demonstrate lipid membrane remodeling in response to local chemical gradients and offer potential insights into the cell membrane behavior under conditions of varying calcium ion concentrations.
AB - Experimental and theoretical studies on ion-lipid interactions predict that binding of calcium ions to cell membranes leads to macroscopic mechanical effects and membrane remodeling. Herein, we provide experimental evidence that a point source of Ca2+ acting upon a negatively charged membrane generates spontaneous curvature and triggers the formation of tubular protrusions that point away from the ion source. This behavior is rationalized by strong binding of the divalent cations to the surface of the charged bilayer, which effectively neutralizes the surface charge density of outer leaflet of the bilayer. The mismatch in the surface charge density of the two leaflets leads to nonzero spontaneous curvature. We probe this mismatch through the use of molecular dynamics simulations and validate that calcium ion binding to a lipid membrane is sufficient to generate inward spontaneous curvature, bending the membrane. Additionally, we demonstrate that the formed tubular protrusions can be translated along the vesicle surface in a controlled manner by repositioning the site of localized Ca2+ exposure. The findings demonstrate lipid membrane remodeling in response to local chemical gradients and offer potential insights into the cell membrane behavior under conditions of varying calcium ion concentrations.
KW - COARSE-GRAINED MODEL
KW - PHOSPHATIDYLSERINE
KW - BILAYER
KW - DYNAMICS
KW - FUSION
KW - CURVATURE
KW - PHOSPHATIDYLCHOLINE
KW - INVAGINATIONS
KW - NANOTUBES
KW - MAGNESIUM
U2 - 10.1021/acs.langmuir.7b01461
DO - 10.1021/acs.langmuir.7b01461
M3 - Journal article
VL - 33
SP - 11010
EP - 11017
JO - Langmuir
JF - Langmuir
SN - 0743-7463
IS - 41
ER -
ID: 316867909