Effect of supercoiling on the λ switch
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Effect of supercoiling on the λ switch. / Norregaard, Kamilla; Andersson, Magnus; Sneppen, Kim; Nielsen, Peter Eigil; Brown, Stanley; Oddershede, Lene B.
I: Bacteriophage, Bind 4, Nr. 2, e27517, 01.01.2014, s. 1-5.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Effect of supercoiling on the λ switch
AU - Norregaard, Kamilla
AU - Andersson, Magnus
AU - Sneppen, Kim
AU - Nielsen, Peter Eigil
AU - Brown, Stanley
AU - Oddershede, Lene B
PY - 2014/1/1
Y1 - 2014/1/1
N2 - The lysogenic state of the λ switch is exceptionally stable, still, it is capable of responding to DNA-damage and rapidly enter the lytic state. We invented an assay where PNA mediated tethering of a plasmid allowed for single molecule investigations of the effect of supercoiling on the efficiency of the epigenetic λ switch. Compared with non-supercoiled DNA, the presence of supercoils enhances the CI-mediated DNA looping probability and renders the transition between the looped and unlooped states steeper, thus increasing the Hill coefficient. Interestingly, the transition occurs exactly at the CI concentration corresponding to the minimum number of CI molecules capable of maintaining the pRM-repressed state. Based on these results we propose that supercoiling maintains the pRM-repressible state as CI concentration decline during induction and thus prevent autoregulation of cI from interfering with induction.
AB - The lysogenic state of the λ switch is exceptionally stable, still, it is capable of responding to DNA-damage and rapidly enter the lytic state. We invented an assay where PNA mediated tethering of a plasmid allowed for single molecule investigations of the effect of supercoiling on the efficiency of the epigenetic λ switch. Compared with non-supercoiled DNA, the presence of supercoils enhances the CI-mediated DNA looping probability and renders the transition between the looped and unlooped states steeper, thus increasing the Hill coefficient. Interestingly, the transition occurs exactly at the CI concentration corresponding to the minimum number of CI molecules capable of maintaining the pRM-repressed state. Based on these results we propose that supercoiling maintains the pRM-repressible state as CI concentration decline during induction and thus prevent autoregulation of cI from interfering with induction.
U2 - 10.4161/bact.27517
DO - 10.4161/bact.27517
M3 - Journal article
C2 - 24386605
VL - 4
SP - 1
EP - 5
JO - Bacteriophage
JF - Bacteriophage
SN - 2159-7073
IS - 2
M1 - e27517
ER -
ID: 108770542