Sall4 and Myocd Empower Direct Cardiac Reprogramming From Adult Cardiac Fibroblasts After Injury
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Sall4 and Myocd Empower Direct Cardiac Reprogramming From Adult Cardiac Fibroblasts After Injury. / Zhao, Hong; Zhang, Yi; Xu, Xiaochan; Sun, Qiushi; Yang, Chunyan; Wang, Hao; Yang, Junbo; Yang, Yang; Yang, Xiaochun; Liu, Yi; Zhao, Yang.
I: Frontiers in Cell and Developmental Biology, Bind 9, 608367, 26.02.2021.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Sall4 and Myocd Empower Direct Cardiac Reprogramming From Adult Cardiac Fibroblasts After Injury
AU - Zhao, Hong
AU - Zhang, Yi
AU - Xu, Xiaochan
AU - Sun, Qiushi
AU - Yang, Chunyan
AU - Wang, Hao
AU - Yang, Junbo
AU - Yang, Yang
AU - Yang, Xiaochun
AU - Liu, Yi
AU - Zhao, Yang
PY - 2021/2/26
Y1 - 2021/2/26
N2 - Direct conversion of fibroblasts into induced cardiomyocytes (iCMs) holds promising potential to generate functional cardiomyocytes for drug development and clinical applications, especially for direct in situ heart regeneration by delivery of reprogramming genes into adult cardiac fibroblasts in injured hearts. For a decade, many cocktails of transcription factors have been developed to generate iCMs from fibroblasts of different tissues in vitro and some were applied in vivo. Here, we aimed to develop genetic cocktails that induce cardiac reprogramming directly in cultured cardiac fibroblasts isolated from adult mice with myocardial infarction (MICFs), which could be more relevant to heart diseases. We found that the widely used genetic cocktail, Gata4, Mef2c, and Tbx5 (GMT) were inefficient in reprogramming cardiomyocytes from MICFs. In a whole well of a 12-well plate, less than 10 mCherry(+) cells (
AB - Direct conversion of fibroblasts into induced cardiomyocytes (iCMs) holds promising potential to generate functional cardiomyocytes for drug development and clinical applications, especially for direct in situ heart regeneration by delivery of reprogramming genes into adult cardiac fibroblasts in injured hearts. For a decade, many cocktails of transcription factors have been developed to generate iCMs from fibroblasts of different tissues in vitro and some were applied in vivo. Here, we aimed to develop genetic cocktails that induce cardiac reprogramming directly in cultured cardiac fibroblasts isolated from adult mice with myocardial infarction (MICFs), which could be more relevant to heart diseases. We found that the widely used genetic cocktail, Gata4, Mef2c, and Tbx5 (GMT) were inefficient in reprogramming cardiomyocytes from MICFs. In a whole well of a 12-well plate, less than 10 mCherry(+) cells (
KW - myofibroblast
KW - cardiac reprogramming
KW - high efficiency
KW - Sall4
KW - Myocd
KW - MICF
U2 - 10.3389/fcell.2021.608367
DO - 10.3389/fcell.2021.608367
M3 - Journal article
C2 - 33718351
VL - 9
JO - Frontiers in Cell and Developmental Biology
JF - Frontiers in Cell and Developmental Biology
SN - 2296-634X
M1 - 608367
ER -
ID: 259825620