RNA preparation and characterization for gene expression studies
Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Undervisning
Much information can be obtained from knowledge of the relative expression level of each gene in the transcriptome. With the current advances in technology as little as a single cell is required as starting material for gene expression experiments. The mRNA from a single cell may be linearly amplified to an excess of 10(6)-fold. Reverse transcription and fluorescent labeling of the amplified RNA yields a stable target for subsequent hybridization to DNA microarrays.
Originalsprog | Engelsk |
---|---|
Titel | Methods in Molecular Biology : DNA Microarrays for Biomedical Research |
Redaktører | Hans Martin Dufva |
Antal sider | 17 |
Vol/bind | 529 |
Udgivelsessted | New York, NY |
Forlag | Springer Science+Business Media |
Publikationsdato | 2009 |
Udgave | 1 |
Sider | 115-132 |
ISBN (Trykt) | 1064-3745, 978-1-934115-69-5 |
ISBN (Elektronisk) | 1940-6029, 978-1-59745-538-1 |
DOI | |
Status | Udgivet - 2009 |
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