Family relationship of female breeders reduce the systematic inter-individual variation in the gut microbiota of inbred laboratory mice
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Family relationship of female breeders reduce the systematic inter-individual variation in the gut microbiota of inbred laboratory mice. / Hufeldt, Majbritt Ravn; Nielsen, Dennis Sandris; Vogensen, Finn Kvist; Midtvedt, T.; Hansen, Axel Jacob Kornerup.
I: Laboratory Animals. Journal of the Laboratory Animal Science Association, Bind 44, Nr. 4, 2010, s. 283-289.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Family relationship of female breeders reduce the systematic inter-individual variation in the gut microbiota of inbred laboratory mice
AU - Hufeldt, Majbritt Ravn
AU - Nielsen, Dennis Sandris
AU - Vogensen, Finn Kvist
AU - Midtvedt, T.
AU - Hansen, Axel Jacob Kornerup
PY - 2010
Y1 - 2010
N2 - The gut microbiota (GM) may influence disease expression in several animal models for inflammatory diseases. It may therefore seem reasonable to pursue reduction in the number of animals used for individual studies by reducing the variation in the GM. Previous studies have shown that the composition of the GM is related to genetics to a certain extent. We hypothesized that the GM similarity in a group of mice born by mothers not being sisters would be lower than that in a group born by mothers being sisters. The lower similarity could lead to clustering of the GM of mice born by non-sisters according to their mothers, while such clustering would not be visible if the mothers were sisters. We used 16S rRNA gene (V3 region) polymerase chain reaction-derived amplicon profiling by denaturing gradient gel electrophoresis (DGGE) to study the GM composition in caecum samples of 33 eight-week-old C57BL/6Sca mice from a breeding set-up with dam breeders that were sisters, as well as caecum samples of 35 eight-week-old C57BL/6Sca mice from a breeding set-up with dam breeders that were not sisters. Principal component analysis revealed a significant difference between the litters from the breeding set-up with dam breeders that were not sisters, whereas no significant difference between the litters based on the breeding set-up with dam breeders that were sisters was observed. The results obtained indicate that the systematic variation in the GM of inbred mice can be reduced by increasing the family relatedness of the breeding pairs.
AB - The gut microbiota (GM) may influence disease expression in several animal models for inflammatory diseases. It may therefore seem reasonable to pursue reduction in the number of animals used for individual studies by reducing the variation in the GM. Previous studies have shown that the composition of the GM is related to genetics to a certain extent. We hypothesized that the GM similarity in a group of mice born by mothers not being sisters would be lower than that in a group born by mothers being sisters. The lower similarity could lead to clustering of the GM of mice born by non-sisters according to their mothers, while such clustering would not be visible if the mothers were sisters. We used 16S rRNA gene (V3 region) polymerase chain reaction-derived amplicon profiling by denaturing gradient gel electrophoresis (DGGE) to study the GM composition in caecum samples of 33 eight-week-old C57BL/6Sca mice from a breeding set-up with dam breeders that were sisters, as well as caecum samples of 35 eight-week-old C57BL/6Sca mice from a breeding set-up with dam breeders that were not sisters. Principal component analysis revealed a significant difference between the litters from the breeding set-up with dam breeders that were not sisters, whereas no significant difference between the litters based on the breeding set-up with dam breeders that were sisters was observed. The results obtained indicate that the systematic variation in the GM of inbred mice can be reduced by increasing the family relatedness of the breeding pairs.
KW - Former LIFE faculty
KW - Laboratory animals
KW - variation of the gut microbiota
KW - Reduction
KW - PCR/DGGE
U2 - 10.1258/la.2010.010058
DO - 10.1258/la.2010.010058
M3 - Journal article
C2 - 20713427
VL - 44
SP - 283
EP - 289
JO - Laboratory Animals
JF - Laboratory Animals
SN - 0023-6772
IS - 4
ER -
ID: 32375174