Network analyses reveal negative link between changes in adipose tissue GDF15 and BMI during dietary induced weight loss
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Network analyses reveal negative link between changes in adipose tissue GDF15 and BMI during dietary induced weight loss. / Imbert, Alyssa; Vialaneix, Nathalie; Marquis, Julien; Vion, Julie; Charpagne, Aline; Metairon, Sylviane; Laurens, Claire; Moro, Cedric; Boulet, Nathalie; Walter, Ondine; Lefebvre, Grégory; Hager, Jörg; Langin, Dominique; Saris, Wim H M; Astrup, Arne; Viguerie, Nathalie; Valsesia, Armand.
I: Journal of Clinical Endocrinology and Metabolism, Bind 107, Nr. 1, 2022, s. e130-e142.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Network analyses reveal negative link between changes in adipose tissue GDF15 and BMI during dietary induced weight loss
AU - Imbert, Alyssa
AU - Vialaneix, Nathalie
AU - Marquis, Julien
AU - Vion, Julie
AU - Charpagne, Aline
AU - Metairon, Sylviane
AU - Laurens, Claire
AU - Moro, Cedric
AU - Boulet, Nathalie
AU - Walter, Ondine
AU - Lefebvre, Grégory
AU - Hager, Jörg
AU - Langin, Dominique
AU - Saris, Wim H M
AU - Astrup, Arne
AU - Viguerie, Nathalie
AU - Valsesia, Armand
N1 - © The Author(s) 2021. Published by Oxford University Press on behalf of the Endocrine Society. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.
PY - 2022
Y1 - 2022
N2 - Context: Adipose tissue (AT) transcriptome studies provide holistic pictures of adaptation to weight and related bioclinical settings changes.Objective: To implement AT gene expression profiling and investigate the link between changes in bioclinical parameters and AT gene expression during three steps of a two-phase dietary intervention (DI).Methods: AT transcriptome profiling was obtained from sequencing 1051 samples, corresponding to 556 distinct individuals enrolled in a weight loss intervention (8-week low calorie diet (LCD) at 800 kcal/d) followed with a 6-month ad libitum randomized DI. Transcriptome profiles obtained with QuantSeq sequencing were benchmarked against Illumina RNAseq. Reverse transcription quantitative polymerase chain reaction was used to further confirm associations. Cell specificity was assessed using freshly isolated cells and THP-1 cell line.Results: During LCD, 5 modules were found, of which 3 included at least 1 bioclinical variable. Change in body mass index (BMI) connected with changes in mRNA level of genes with inflammatory response signature. In this module, change in BMI was negatively associated to changes in expression of genes encoding secreted protein (GDF15, CCL3, and SPP1). Through all phases of the DI, change in GDF15 was connected to changes in SPP1, CCL3, LIPA and CD68. Further characterization showed that these genes were specific to macrophages (with LIPA, CD68 and GDF15 expressed in anti-inflammatory macrophages) and GDF15 also expressed in preadipocytes.Conclusion: Network analyses identified a novel AT feature with GDF15 upregulated with calorie restriction induced weight loss, concomitantly to macrophage markers. In AT, GDF15 was expressed in preadipocytes and macrophages where it was a hallmark of anti-inflammatory cells.
AB - Context: Adipose tissue (AT) transcriptome studies provide holistic pictures of adaptation to weight and related bioclinical settings changes.Objective: To implement AT gene expression profiling and investigate the link between changes in bioclinical parameters and AT gene expression during three steps of a two-phase dietary intervention (DI).Methods: AT transcriptome profiling was obtained from sequencing 1051 samples, corresponding to 556 distinct individuals enrolled in a weight loss intervention (8-week low calorie diet (LCD) at 800 kcal/d) followed with a 6-month ad libitum randomized DI. Transcriptome profiles obtained with QuantSeq sequencing were benchmarked against Illumina RNAseq. Reverse transcription quantitative polymerase chain reaction was used to further confirm associations. Cell specificity was assessed using freshly isolated cells and THP-1 cell line.Results: During LCD, 5 modules were found, of which 3 included at least 1 bioclinical variable. Change in body mass index (BMI) connected with changes in mRNA level of genes with inflammatory response signature. In this module, change in BMI was negatively associated to changes in expression of genes encoding secreted protein (GDF15, CCL3, and SPP1). Through all phases of the DI, change in GDF15 was connected to changes in SPP1, CCL3, LIPA and CD68. Further characterization showed that these genes were specific to macrophages (with LIPA, CD68 and GDF15 expressed in anti-inflammatory macrophages) and GDF15 also expressed in preadipocytes.Conclusion: Network analyses identified a novel AT feature with GDF15 upregulated with calorie restriction induced weight loss, concomitantly to macrophage markers. In AT, GDF15 was expressed in preadipocytes and macrophages where it was a hallmark of anti-inflammatory cells.
KW - Faculty of Science
KW - Adipocyte
KW - Macrophage
KW - Weight loss
KW - Network analyses
KW - Low calorie diet
KW - Inflammation
U2 - 10.1210/clinem/dgab621
DO - 10.1210/clinem/dgab621
M3 - Journal article
C2 - 34415992
VL - 107
SP - e130-e142
JO - Journal of Clinical Endocrinology and Metabolism
JF - Journal of Clinical Endocrinology and Metabolism
SN - 0021-972X
IS - 1
ER -
ID: 276462418