Cytokine release from human peripheral blood leucocytes incubated with endotoxin with and without prior infection with influenza virus: relevance to the sudden infant death syndrome
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Cytokine release from human peripheral blood leucocytes incubated with endotoxin with and without prior infection with influenza virus : relevance to the sudden infant death syndrome. / Banner, Jytte; Smith, H; Sweet, C.
I: International Journal of Experimental Pathology, Bind 74, Nr. 3, 06.1993, s. 291-7.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Cytokine release from human peripheral blood leucocytes incubated with endotoxin with and without prior infection with influenza virus
T2 - relevance to the sudden infant death syndrome
AU - Banner, Jytte
AU - Smith, H
AU - Sweet, C
PY - 1993/6
Y1 - 1993/6
N2 - Previous work with a neonatal ferret model for human SIDS had indicated that inflammation caused by a combination of influenza virus and bacterial endotoxin may be a cause of human SIDS. To determine whether cytokines may be involved in this inflammatory response, levels of interleukin (IL)-1 beta, IL-6 and tumour necrosis factor (TNF)-alpha were examined, using ELISA assays, in culture supernatants of human peripheral blood leucocytes infected with influenza virus and subsequently incubated with endotoxin. Levels of TNF-alpha were increased compared to cells incubated with virus or endotoxin alone. Levels of IL-1 beta were also increased whereas levels of IL-6 were generally not enhanced. Cytokines appeared within 1-2 h of stimulation with virus or endotoxin and increased subsequently to reach maximum titres between 16 and 20 h post treatment. While levels of cytokine were much lower when determined using bioassays rather than ELISA assays, the pattern of increased yields from cells incubated with virus and endotoxin compared with either alone was still evident. The possible importance of these observations for SIDS victims is discussed.
AB - Previous work with a neonatal ferret model for human SIDS had indicated that inflammation caused by a combination of influenza virus and bacterial endotoxin may be a cause of human SIDS. To determine whether cytokines may be involved in this inflammatory response, levels of interleukin (IL)-1 beta, IL-6 and tumour necrosis factor (TNF)-alpha were examined, using ELISA assays, in culture supernatants of human peripheral blood leucocytes infected with influenza virus and subsequently incubated with endotoxin. Levels of TNF-alpha were increased compared to cells incubated with virus or endotoxin alone. Levels of IL-1 beta were also increased whereas levels of IL-6 were generally not enhanced. Cytokines appeared within 1-2 h of stimulation with virus or endotoxin and increased subsequently to reach maximum titres between 16 and 20 h post treatment. While levels of cytokine were much lower when determined using bioassays rather than ELISA assays, the pattern of increased yields from cells incubated with virus and endotoxin compared with either alone was still evident. The possible importance of these observations for SIDS victims is discussed.
KW - Cells, Cultured
KW - Cytokines
KW - Dose-Response Relationship, Immunologic
KW - Endotoxins
KW - Humans
KW - Infant
KW - Influenza A virus
KW - Interleukin-1
KW - Interleukin-6
KW - Leukocytes
KW - Orthomyxoviridae Infections
KW - Sudden Infant Death
KW - Time Factors
KW - Tumor Necrosis Factor-alpha
M3 - Journal article
C2 - 8392861
VL - 74
SP - 291
EP - 297
JO - International Journal of Experimental Pathology
JF - International Journal of Experimental Pathology
SN - 0959-9673
IS - 3
ER -
ID: 46810128