Mimicking the Mammalian Plasma Membrane: An Overview of Lipid Membrane Models for Biophysical Studies
Publikation: Bidrag til tidsskrift › Review › Forskning › fagfællebedømt
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Mimicking the Mammalian Plasma Membrane : An Overview of Lipid Membrane Models for Biophysical Studies. / Luchini, Alessandra; Vitiello, Giuseppe.
I: Biomimetics, Bind 6, Nr. 1, 3, 03.2021.Publikation: Bidrag til tidsskrift › Review › Forskning › fagfællebedømt
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TY - JOUR
T1 - Mimicking the Mammalian Plasma Membrane
T2 - An Overview of Lipid Membrane Models for Biophysical Studies
AU - Luchini, Alessandra
AU - Vitiello, Giuseppe
PY - 2021/3
Y1 - 2021/3
N2 - Cell membranes are very complex biological systems including a large variety of lipids and proteins. Therefore, they are difficult to extract and directly investigate with biophysical methods. For many decades, the characterization of simpler biomimetic lipid membranes, which contain only a few lipid species, provided important physico-chemical information on the most abundant lipid species in cell membranes. These studies described physical and chemical properties that are most likely similar to those of real cell membranes. Indeed, biomimetic lipid membranes can be easily prepared in the lab and are compatible with multiple biophysical techniques. Lipid phase transitions, the bilayer structure, the impact of cholesterol on the structure and dynamics of lipid bilayers, and the selective recognition of target lipids by proteins, peptides, and drugs are all examples of the detailed information about cell membranes obtained by the investigation of biomimetic lipid membranes. This review focuses specifically on the advances that were achieved during the last decade in the field of biomimetic lipid membranes mimicking the mammalian plasma membrane. In particular, we provide a description of the most common types of lipid membrane models used for biophysical characterization, i.e., lipid membranes in solution and on surfaces, as well as recent examples of their applications for the investigation of protein-lipid and drug-lipid interactions. Altogether, promising directions for future developments of biomimetic lipid membranes are the further implementation of natural lipid mixtures for the development of more biologically relevant lipid membranes, as well as the development of sample preparation protocols that enable the incorporation of membrane proteins in the biomimetic lipid membranes.
AB - Cell membranes are very complex biological systems including a large variety of lipids and proteins. Therefore, they are difficult to extract and directly investigate with biophysical methods. For many decades, the characterization of simpler biomimetic lipid membranes, which contain only a few lipid species, provided important physico-chemical information on the most abundant lipid species in cell membranes. These studies described physical and chemical properties that are most likely similar to those of real cell membranes. Indeed, biomimetic lipid membranes can be easily prepared in the lab and are compatible with multiple biophysical techniques. Lipid phase transitions, the bilayer structure, the impact of cholesterol on the structure and dynamics of lipid bilayers, and the selective recognition of target lipids by proteins, peptides, and drugs are all examples of the detailed information about cell membranes obtained by the investigation of biomimetic lipid membranes. This review focuses specifically on the advances that were achieved during the last decade in the field of biomimetic lipid membranes mimicking the mammalian plasma membrane. In particular, we provide a description of the most common types of lipid membrane models used for biophysical characterization, i.e., lipid membranes in solution and on surfaces, as well as recent examples of their applications for the investigation of protein-lipid and drug-lipid interactions. Altogether, promising directions for future developments of biomimetic lipid membranes are the further implementation of natural lipid mixtures for the development of more biologically relevant lipid membranes, as well as the development of sample preparation protocols that enable the incorporation of membrane proteins in the biomimetic lipid membranes.
KW - mammalian plasma membrane
KW - biomimetic lipid membranes
KW - biomimicking models
KW - protein-membrane interactions
KW - drug-membrane interactions
KW - I GLYCOPROTEINS B
KW - PROTEIN
KW - IBUPROFEN
U2 - 10.3390/biomimetics6010003
DO - 10.3390/biomimetics6010003
M3 - Review
C2 - 33396534
VL - 6
JO - Biomimetics
JF - Biomimetics
SN - 2313-7673
IS - 1
M1 - 3
ER -
ID: 259825227