Localization of thymosin ß-4 in tumors
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Localization of thymosin ß-4 in tumors. / Larsson, Lars-Inge; Holck, Susanne.
I: Annals of the New York Academy of Sciences, Bind 1112, 2007, s. 317-325.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Localization of thymosin ß-4 in tumors
AU - Larsson, Lars-Inge
AU - Holck, Susanne
N1 - Thymosins in Health and Disease First International Symposium
PY - 2007
Y1 - 2007
N2 - Overexpression of thymosin ß-4 has been linked to malignant progression but the localization of this polypeptide within tumor is incompletely known. We therefore examined breast cancers for thymosin ß-4 using immunofluorescence. Reactive cells were identified with monoclonal cell marker antibodies. A very heterogeneous staining pattern for thymosin ß-4 was observed. Thus, while leukocytes and macrophages showed intense reactivity for this polypeptide, cancer cells, and endothelial cells showed a much more variable reactivity. A similar heterogeneous staining was observed also in colorectal carcinomas. The degree of staining of breast cancer cells for thymosin ß-4 correlated neither to histological grade nor to endothelial cell staining. However, therewas a tendency toward correlation (P = 0.07) between staining of endothelial cells and histological grade. Treatment of cultured breast cancer cells (SK-BR-3) with 1-4 µg thymosin ß-4/mL significantly increased cell numbers, as determined by MTT-assays. These data reveal an unexpected cellular heterogeneity of thymosin ß-4 expression in breast and colonic carcinomas and suggest that local release of this polypeptide in the tumor microenvironment may modulate tumor behavior.
AB - Overexpression of thymosin ß-4 has been linked to malignant progression but the localization of this polypeptide within tumor is incompletely known. We therefore examined breast cancers for thymosin ß-4 using immunofluorescence. Reactive cells were identified with monoclonal cell marker antibodies. A very heterogeneous staining pattern for thymosin ß-4 was observed. Thus, while leukocytes and macrophages showed intense reactivity for this polypeptide, cancer cells, and endothelial cells showed a much more variable reactivity. A similar heterogeneous staining was observed also in colorectal carcinomas. The degree of staining of breast cancer cells for thymosin ß-4 correlated neither to histological grade nor to endothelial cell staining. However, therewas a tendency toward correlation (P = 0.07) between staining of endothelial cells and histological grade. Treatment of cultured breast cancer cells (SK-BR-3) with 1-4 µg thymosin ß-4/mL significantly increased cell numbers, as determined by MTT-assays. These data reveal an unexpected cellular heterogeneity of thymosin ß-4 expression in breast and colonic carcinomas and suggest that local release of this polypeptide in the tumor microenvironment may modulate tumor behavior.
KW - Former LIFE faculty
KW - thomosin ß-4
KW - cancer
KW - colorectal
KW - breast
KW - endothelial cells
KW - macrophages
U2 - 10.1196/annals.1415.005
DO - 10.1196/annals.1415.005
M3 - Journal article
C2 - 17495241
VL - 1112
SP - 317
EP - 325
JO - Annals of The Lyceum of Natural History of New York
JF - Annals of The Lyceum of Natural History of New York
SN - 0077-8923
ER -
ID: 8081204