MAO-A inhibitory activity of quercetin from Calluna vulgaris (L.) Hull
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MAO-A inhibitory activity of quercetin from Calluna vulgaris (L.) Hull. / Saaby, Lasse; Rasmussen, Hasse Bonde; Jäger, Anna Katharina.
I: Journal of Ethnopharmacology, Bind 121, Nr. 1, 2009, s. 178-181.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - MAO-A inhibitory activity of quercetin from Calluna vulgaris (L.) Hull
AU - Saaby, Lasse
AU - Rasmussen, Hasse Bonde
AU - Jäger, Anna Katharina
N1 - Keywords: Calluna Vulgaris; Eriaceae; Monoamine oxidase A inhibitor; Quercetin; Traditional medicine
PY - 2009
Y1 - 2009
N2 - AIM OF THE STUDY: This study investigated MAO-A inhibitory activity of methanol extract of Calluna vulgaris (L.) Hull., which traditionally has been used as a nerve calming remedy. MATERIALS AND METHODS: A methanolic extract of Calluna vulgaris was partitioned against heptane, ethyl acetate and water. The three fractions were tested in a photometric peroxidase linked MAO-A bioassay. The ethyl acetate phase showed the highest MAO-A inhibitory activity. Quercetin was isolated by VLC through bioassay-guided fractionation and purified by re-crystallisation. The structure was elucidated by LC-MS and (1)H NMR. RESULTS: The IC(50)-value for MAO-A inhibition by quercetin was 18+/-0.2muM in an assay where the IC(50)-value for MAO-A inhibition by clorgylin was 0.2+/-0.02muM. CONCLUSION: The content of quercetin in Calluna vulgaris might explain the reported nerve calming effect of the plant.
AB - AIM OF THE STUDY: This study investigated MAO-A inhibitory activity of methanol extract of Calluna vulgaris (L.) Hull., which traditionally has been used as a nerve calming remedy. MATERIALS AND METHODS: A methanolic extract of Calluna vulgaris was partitioned against heptane, ethyl acetate and water. The three fractions were tested in a photometric peroxidase linked MAO-A bioassay. The ethyl acetate phase showed the highest MAO-A inhibitory activity. Quercetin was isolated by VLC through bioassay-guided fractionation and purified by re-crystallisation. The structure was elucidated by LC-MS and (1)H NMR. RESULTS: The IC(50)-value for MAO-A inhibition by quercetin was 18+/-0.2muM in an assay where the IC(50)-value for MAO-A inhibition by clorgylin was 0.2+/-0.02muM. CONCLUSION: The content of quercetin in Calluna vulgaris might explain the reported nerve calming effect of the plant.
KW - Former Faculty of Pharmaceutical Sciences
U2 - 10.1016/j.jep.2008.10.012
DO - 10.1016/j.jep.2008.10.012
M3 - Journal article
C2 - 19013512
VL - 121
SP - 178
EP - 181
JO - Journal of Ethnopharmacology
JF - Journal of Ethnopharmacology
SN - 0378-8741
IS - 1
ER -
ID: 9509860