Pathology and localization of Avibacterium endocarditidis in experimentally infected broiler breeders
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Pathology and localization of Avibacterium endocarditidis in experimentally infected broiler breeders. / Møller, B. Horne; Bisgaard, Magne; Pors, Susanne Elisabeth.
I: Journal of Comparative Pathology, Bind 150, Nr. 2-3, 2014, s. 266-275.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Pathology and localization of Avibacterium endocarditidis in experimentally infected broiler breeders
AU - Møller, B. Horne
AU - Bisgaard, Magne
AU - Pors, Susanne Elisabeth
N1 - Copyright © 2013 Elsevier Ltd. All rights reserved.
PY - 2014
Y1 - 2014
N2 - The aim of this study was to investigate the pathogenic potential of Avibacterium endocarditidis. Forty broiler breeders were inoculated with one of three doses of the organism and killed at different time points. Bacteriology, pathology and fluorescence in-situ hybridization (FISH) were performed to evaluate bacterial growth and the development of lesions. Abundant growth in pure culture of A. endocarditidis was always obtained from valvular lesions, while only poor growth or no growth was obtained from liver and spleen lesions, confirming previous observations from naturally occurring cases. Gross lesions and histopathological findings confirmed previous observations. Valvular lesions were acute, ranging from slight thickening of the valves to severe inflammation. In most cases, bacteria colonized the valves. Lesions observed in the spleen included different degrees of necrosis and liver lesions ranged from very small infarcts to large areas of coagulative necrosis. Arthritis occurred in 19 birds, 15 of which tested positive for A. endocarditidis. Most birds developed bacteraemia, but the inability to isolate bacteria from the liver and spleen and the lack of bacteria demonstrated by FISH and histopathology, suggested lack of fulminant septicaemia. A significant correlation between the size of dose inoculated and development of valvular endocarditis was not observed; however, regressive changes in the ovary, liver necrosis and hepato-, spleno- and renomegaly were significantly dose dependent. A. endocarditidis represents a potential pathogen for chickens, the reservoir of which remains to be determined.
AB - The aim of this study was to investigate the pathogenic potential of Avibacterium endocarditidis. Forty broiler breeders were inoculated with one of three doses of the organism and killed at different time points. Bacteriology, pathology and fluorescence in-situ hybridization (FISH) were performed to evaluate bacterial growth and the development of lesions. Abundant growth in pure culture of A. endocarditidis was always obtained from valvular lesions, while only poor growth or no growth was obtained from liver and spleen lesions, confirming previous observations from naturally occurring cases. Gross lesions and histopathological findings confirmed previous observations. Valvular lesions were acute, ranging from slight thickening of the valves to severe inflammation. In most cases, bacteria colonized the valves. Lesions observed in the spleen included different degrees of necrosis and liver lesions ranged from very small infarcts to large areas of coagulative necrosis. Arthritis occurred in 19 birds, 15 of which tested positive for A. endocarditidis. Most birds developed bacteraemia, but the inability to isolate bacteria from the liver and spleen and the lack of bacteria demonstrated by FISH and histopathology, suggested lack of fulminant septicaemia. A significant correlation between the size of dose inoculated and development of valvular endocarditis was not observed; however, regressive changes in the ovary, liver necrosis and hepato-, spleno- and renomegaly were significantly dose dependent. A. endocarditidis represents a potential pathogen for chickens, the reservoir of which remains to be determined.
KW - Faculty of Health and Medical Sciences
KW - Avibacterium endocarditidis
KW - poultry
KW - valvular endocarditis
U2 - 10.1016/j.jcpa.2013.07.006
DO - 10.1016/j.jcpa.2013.07.006
M3 - Journal article
C2 - 24016779
VL - 150
SP - 266
EP - 275
JO - Journal of Comparative Pathology
JF - Journal of Comparative Pathology
SN - 0021-9975
IS - 2-3
ER -
ID: 50467073