Genetic and sexual separation between insect resistant and susceptible Barbarea vulgaris plants in Denmark
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Genetic and sexual separation between insect resistant and susceptible Barbarea vulgaris plants in Denmark. / Toneatto, Fiorello; Nielsen, Jens Kvist; Ørgaard, Marian; Hauser, Thure Pavlo.
I: Molecular Ecology, Bind 19, Nr. 16, 2010, s. 3456-3465.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Genetic and sexual separation between insect resistant and susceptible Barbarea vulgaris plants in Denmark
AU - Toneatto, Fiorello
AU - Nielsen, Jens Kvist
AU - Ørgaard, Marian
AU - Hauser, Thure Pavlo
PY - 2010
Y1 - 2010
N2 - Co-evolution between herbivores and plants is believed to be one of the processes creating Earth’s biodiversity. However, it is difficult to disentangle to what extent diversification is really driven by herbivores or by other historical-geographical processes like allopatric isolation. In the cruciferous plant Barbarea vulgaris, some Danish individuals are resistant to herbivory by flea beetles (Phyllotreta nemorum), whereas others are not. The flea beetles are, in parallel, either resistant or susceptible to the plants defenses. To understand the historical-evolutionary framework of these interactions, we tested how genetically divergent resistant and susceptible plants are, using microsatellite markers. To test whether they are reproductively fully compatible, resistant and susceptible plants were grown intermixed in an outdoor experiment, and the paternity of open-pollinated offspring was determined by analysis of molecular markers. Resistant and susceptible Danish plants were genetically strongly differentiated and produced significantly fewer hybrids than expected from random mating or nearest neighbour mating. Our results suggest that the two types belong to different evolutionary lineages that have been (partly) isolated at some time, during which genetic and reproductive divergence evolved. A parsimonious scenario could be that the two plant types were isolated in different refugia during the previous ice age, from which they migrated into and met in Denmark and possibly neighbouring regions. If so, resistance and susceptibility has for unknown reasons become associated with the different evolutionary lineages.
AB - Co-evolution between herbivores and plants is believed to be one of the processes creating Earth’s biodiversity. However, it is difficult to disentangle to what extent diversification is really driven by herbivores or by other historical-geographical processes like allopatric isolation. In the cruciferous plant Barbarea vulgaris, some Danish individuals are resistant to herbivory by flea beetles (Phyllotreta nemorum), whereas others are not. The flea beetles are, in parallel, either resistant or susceptible to the plants defenses. To understand the historical-evolutionary framework of these interactions, we tested how genetically divergent resistant and susceptible plants are, using microsatellite markers. To test whether they are reproductively fully compatible, resistant and susceptible plants were grown intermixed in an outdoor experiment, and the paternity of open-pollinated offspring was determined by analysis of molecular markers. Resistant and susceptible Danish plants were genetically strongly differentiated and produced significantly fewer hybrids than expected from random mating or nearest neighbour mating. Our results suggest that the two types belong to different evolutionary lineages that have been (partly) isolated at some time, during which genetic and reproductive divergence evolved. A parsimonious scenario could be that the two plant types were isolated in different refugia during the previous ice age, from which they migrated into and met in Denmark and possibly neighbouring regions. If so, resistance and susceptibility has for unknown reasons become associated with the different evolutionary lineages.
KW - Former LIFE faculty
KW - evolutionary divergence, insect herbivory, paternity, resistance, sexual incompatibility
U2 - 10.1111/j.1365-294X.2010.04760.x
DO - 10.1111/j.1365-294X.2010.04760.x
M3 - Journal article
VL - 19
SP - 3456
EP - 3465
JO - Molecular Ecology
JF - Molecular Ecology
SN - 0962-1083
IS - 16
ER -
ID: 34495641