Improved synthesis methods of standards used for quantitative determination of total isothiocyanates from broccoli in human urine
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
A well-known method for quantification of isothiocyanates (ITCs) and their metabolites is the condensation reaction with 1,2-benzenedithiole to produce 1,3-benzodithiole-2-thione, which can be quantified by high-performance liquid chromatography. Standards of an ITC metabolite and 1,3-benzodithiole-2-thione are required for this assay but are not commercially available. In the present study, we report on an improved synthesisof the ITC metabolite N-acetyl-S-(N-4-methylsulfinylbutylthiocarbamoyl)-l-cysteine and 1,3-benzodithiole-2-thione. The standards were used to quantify the urinary excretion of ITCs from 10 healthy subjects who consumed 350 g broccoli. The excretion was investigated throughout 48 h showing a cumulative urinary ITC excretion of 49.1±25.2% of the dose.
Originalsprog | Engelsk |
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Tidsskrift | Journal of Chromatography - B |
Vol/bind | 852 |
Udgave nummer | 1-2 |
Sider (fra-til) | 229-234 |
Antal sider | 6 |
ISSN | 1570-0232 |
DOI | |
Status | Udgivet - 2007 |
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ID: 8070819