Measurement of skeletal muscle glucose uptake in mice in response to acute treadmill running
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Measurement of skeletal muscle glucose uptake in mice in response to acute treadmill running. / Møller, Lisbeth Liliendal Valbjørn; Raun, Steffen Henning; Fritzen, Andreas Mæchel; Sylow, Lykke.
I: Journal of Biological Methods, Bind 9, Nr. 3, e162, 2022.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Measurement of skeletal muscle glucose uptake in mice in response to acute treadmill running
AU - Møller, Lisbeth Liliendal Valbjørn
AU - Raun, Steffen Henning
AU - Fritzen, Andreas Mæchel
AU - Sylow, Lykke
N1 - CURIS 2022 NEXS 248
PY - 2022
Y1 - 2022
N2 - Skeletal muscle contractions stimulate glucose uptake into the working muscles during exercise. Because this signaling pathway is independent of insulin, exercise constitutes an important alternative pathway to increase glucose uptake, also in insulin-resistant muscle. Therefore, much effort is being put into understanding the molecular regulation of exercise-stimulated glucose uptake by skeletal muscle. To delineate the causal molecular mechanisms whereby muscle contraction or exercise regulate glucose uptake, the investigation of genetically manipulated rodents is necessary. Presented here is a modified and optimized protocol assessing exercise-induced muscle glucose uptake in mice in response to acute treadmill running. Using this high-throughput protocol, running capacity can accurately and reproducibly be determined in mice, and basal- and exercise-stimulated skeletal muscle glucose uptake and intracellular signaling can precisely and dose-dependently be measured in awake mice in vivo without the need for catheterization and with minimal loss of blood.
AB - Skeletal muscle contractions stimulate glucose uptake into the working muscles during exercise. Because this signaling pathway is independent of insulin, exercise constitutes an important alternative pathway to increase glucose uptake, also in insulin-resistant muscle. Therefore, much effort is being put into understanding the molecular regulation of exercise-stimulated glucose uptake by skeletal muscle. To delineate the causal molecular mechanisms whereby muscle contraction or exercise regulate glucose uptake, the investigation of genetically manipulated rodents is necessary. Presented here is a modified and optimized protocol assessing exercise-induced muscle glucose uptake in mice in response to acute treadmill running. Using this high-throughput protocol, running capacity can accurately and reproducibly be determined in mice, and basal- and exercise-stimulated skeletal muscle glucose uptake and intracellular signaling can precisely and dose-dependently be measured in awake mice in vivo without the need for catheterization and with minimal loss of blood.
KW - Faculty of Science
KW - AMPK
KW - Exercise
KW - Glucose uptake
KW - Muscle
KW - Muscle contraction
U2 - 10.14440/jbm.2022.385
DO - 10.14440/jbm.2022.385
M3 - Journal article
C2 - 36404875
VL - 9
JO - Journal of Biological Methods
JF - Journal of Biological Methods
SN - 2326-9901
IS - 3
M1 - e162
ER -
ID: 322893120