Polyamines in spermatocytes and residual bodies of rat testis
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Polyamines in spermatocytes and residual bodies of rat testis. / Shin, Masashi; Larsson, Lars-Inge; Fujiwara, Kunio.
I: Histochemistry and Cell Biology, Bind 127, Nr. 6, 2007, s. 649-655.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Polyamines in spermatocytes and residual bodies of rat testis
AU - Shin, Masashi
AU - Larsson, Lars-Inge
AU - Fujiwara, Kunio
PY - 2007
Y1 - 2007
N2 - Immunocytochemistry for polyamines in the rat testis revealed intense staining of small bodies close to the lumen of seminiferous tubules of spermatogenic stage VII and VIII as well as of spermatocytes. Methyl green-pyronin and proppidium iodide staining combined with DNase or RNase predigestion showed that the small bodies contained RNA, but not DNA and double fluorescence staining showed that polyamines (PAs) colocalized with RNA in the bodies. Electron microscopy confirmed the absence of nuclei in the bodies and revealed that PA immunoreactivity was associated with ribosomes. These results strongly suggest that the small bodies correspond to the residual bodies, and agree with previous results showing localization of PAs to ribosomes in neurons and gastrointestinal epithelial cells. The accumulation of PAs in residual bodies may reflect a termination of their role in spermiogenesis with respect to protein synthesis.
AB - Immunocytochemistry for polyamines in the rat testis revealed intense staining of small bodies close to the lumen of seminiferous tubules of spermatogenic stage VII and VIII as well as of spermatocytes. Methyl green-pyronin and proppidium iodide staining combined with DNase or RNase predigestion showed that the small bodies contained RNA, but not DNA and double fluorescence staining showed that polyamines (PAs) colocalized with RNA in the bodies. Electron microscopy confirmed the absence of nuclei in the bodies and revealed that PA immunoreactivity was associated with ribosomes. These results strongly suggest that the small bodies correspond to the residual bodies, and agree with previous results showing localization of PAs to ribosomes in neurons and gastrointestinal epithelial cells. The accumulation of PAs in residual bodies may reflect a termination of their role in spermiogenesis with respect to protein synthesis.
KW - Former LIFE faculty
KW - Polyamines
KW - Immunocytochemistry
KW - Residual body
KW - Double immunofluorescence
KW - Rat
U2 - 10.1007/s00418-007-0285-7
DO - 10.1007/s00418-007-0285-7
M3 - Journal article
C2 - 17443339
VL - 127
SP - 649
EP - 655
JO - Histochemistry and Cell Biology
JF - Histochemistry and Cell Biology
SN - 0948-6143
IS - 6
ER -
ID: 8078480