Porcine ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1/CD203a): cloning, transcription, expression, mapping, and identification of an NPP1/CD203a epitope for swine workshop cluster 9 (SWC9) monoclonal antibodies
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Porcine ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1/CD203a) : cloning, transcription, expression, mapping, and identification of an NPP1/CD203a epitope for swine workshop cluster 9 (SWC9) monoclonal antibodies. / Petersen, Cathrine Bie; Hillig, Ann-Britt Nygaard; Viuff, Birgitte; Fredholm, Merete; Aasted, Bent; Salomonsen, Jan.
I: Developmental & Comparative Immunology, Bind 31, Nr. 6, 2007, s. 618-631.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Porcine ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1/CD203a)
T2 - cloning, transcription, expression, mapping, and identification of an NPP1/CD203a epitope for swine workshop cluster 9 (SWC9) monoclonal antibodies
AU - Petersen, Cathrine Bie
AU - Hillig, Ann-Britt Nygaard
AU - Viuff, Birgitte
AU - Fredholm, Merete
AU - Aasted, Bent
AU - Salomonsen, Jan
PY - 2007
Y1 - 2007
N2 - Swine workshop cluster 9 (SWC9) antibody identifying a porcien epitope on macrophages and thymocytes was used to precipitate and characterize the molecule from biotinylated macrophages and to obtain peptide sequence by mass spectrometry. The protein was identified as ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1/CD203a). The porcine NPP1/CD203a encoding gene was mapped to chromosome 1 using a radiation hybrid panel, and transcription was investigated by RT-PCR analysis of several tissues. The cDNA was cloned and introduced into COS7 cells resulting in expression of functionally active enzyme and verification of the specificity of an SWC9 reacting monoclonal antibody. The antibody was used for immunohistochemical examination of various porcine tissues. Most prominent expression of NPP1/CD203a was found in lung macrophages and liver sinusoids.
AB - Swine workshop cluster 9 (SWC9) antibody identifying a porcien epitope on macrophages and thymocytes was used to precipitate and characterize the molecule from biotinylated macrophages and to obtain peptide sequence by mass spectrometry. The protein was identified as ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1/CD203a). The porcine NPP1/CD203a encoding gene was mapped to chromosome 1 using a radiation hybrid panel, and transcription was investigated by RT-PCR analysis of several tissues. The cDNA was cloned and introduced into COS7 cells resulting in expression of functionally active enzyme and verification of the specificity of an SWC9 reacting monoclonal antibody. The antibody was used for immunohistochemical examination of various porcine tissues. Most prominent expression of NPP1/CD203a was found in lung macrophages and liver sinusoids.
KW - Former LIFE faculty
KW - SWC9
KW - NPP1
KW - Swine
KW - Macrophage
U2 - 10.1016/j.dci.2006.08.012
DO - 10.1016/j.dci.2006.08.012
M3 - Journal article
C2 - 17064772
VL - 31
SP - 618
EP - 631
JO - Developmental and Comparative Immunology
JF - Developmental and Comparative Immunology
SN - 0145-305X
IS - 6
ER -
ID: 8043334