Regulatory T cells in human immunodeficiency virus-infected patients are elevated and independent of immunological and virological status, as well as initiation of highly active anti-retroviral therapy
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Regulatory T cells in human immunodeficiency virus-infected patients are elevated and independent of immunological and virological status, as well as initiation of highly active anti-retroviral therapy. / Gaardbo, J.C.; Nielsen, S.D.; Vedel, S.J.; Ersbøll, Annette Kjær; Harritshøj, Lene Holm; Ryder, Lars P.; Nielsen, J.O.; Kolte, J.; Gaardbo, J C; Nielsen, Susanne Dam; Vedel, Signe Juul; Ersbøll, A K; Harritshøj, L; Ryder, L P; Nielsen, Jens Ole; Kolte, Lilian.
I: Clinical and Experimental Immunology, Bind 154, Nr. 1, 2008, s. 80-6.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Regulatory T cells in human immunodeficiency virus-infected patients are elevated and independent of immunological and virological status, as well as initiation of highly active anti-retroviral therapy
AU - Gaardbo, J.C.
AU - Nielsen, S.D.
AU - Vedel, S.J.
AU - Ersbøll, Annette Kjær
AU - Harritshøj, Lene Holm
AU - Ryder, Lars P.
AU - Nielsen, J.O.
AU - Kolte, J.
AU - Gaardbo, J C
AU - Nielsen, Susanne Dam
AU - Vedel, Signe Juul
AU - Ersbøll, A K
AU - Harritshøj, L
AU - Ryder, L P
AU - Nielsen, Jens Ole
AU - Kolte, Lilian
PY - 2008
Y1 - 2008
N2 - Infection with human immunodeficiency virus (HIV) causes a dysregulation of the immune system. This is caused by HIV-specific as well as non-specific mechanisms and has not been explained fully. In particular, knowledge is lacking about the potential role of host-mediated immunosuppressive mechanisms. During recent years it has become evident that a subpopulation of T cells [T regulatory (T(regs))] play a major role in sustaining tolerance to self-antigens. To investigate the influence of initiation of highly active anti-retroviral therapy (HAART) on the T(reg) level in HIV-infected patients we have conducted a prospective study enrolling treatment-naive HIV-infected patients just prior to starting treatment with HAART, measuring levels of T(regs) by flow cytometry and mRNA expression of forkhead box P3 (FoxP3) at weeks 0, 4, 12 and 24 of treatment. In this prospective study neither the percentage of CD4(+)CD25(high+) nor the expression of FoxP3 changed significantly during 24 weeks of HAART. Furthermore, HIV patients have higher T(regs) measured as percentages of CD4(+)CD25(high+) cells paralleled by higher levels of FoxP3 compared with healthy controls. The elevated level of T(regs) was found to be independent of both immunological and virological status, indicating that initiation of HAART has minor effects on the T(reg) level in HIV-infected patients.
AB - Infection with human immunodeficiency virus (HIV) causes a dysregulation of the immune system. This is caused by HIV-specific as well as non-specific mechanisms and has not been explained fully. In particular, knowledge is lacking about the potential role of host-mediated immunosuppressive mechanisms. During recent years it has become evident that a subpopulation of T cells [T regulatory (T(regs))] play a major role in sustaining tolerance to self-antigens. To investigate the influence of initiation of highly active anti-retroviral therapy (HAART) on the T(reg) level in HIV-infected patients we have conducted a prospective study enrolling treatment-naive HIV-infected patients just prior to starting treatment with HAART, measuring levels of T(regs) by flow cytometry and mRNA expression of forkhead box P3 (FoxP3) at weeks 0, 4, 12 and 24 of treatment. In this prospective study neither the percentage of CD4(+)CD25(high+) nor the expression of FoxP3 changed significantly during 24 weeks of HAART. Furthermore, HIV patients have higher T(regs) measured as percentages of CD4(+)CD25(high+) cells paralleled by higher levels of FoxP3 compared with healthy controls. The elevated level of T(regs) was found to be independent of both immunological and virological status, indicating that initiation of HAART has minor effects on the T(reg) level in HIV-infected patients.
KW - Former LIFE faculty
KW - flow cytometry/FACS
KW - highly active anti-retroviral therapy
KW - HIV
KW - immune regulation
U2 - 10.1111/j.1365-2249.2008.03725.x
DO - 10.1111/j.1365-2249.2008.03725.x
M3 - Journal article
C2 - 18821942
VL - 154
SP - 80
EP - 86
JO - Clinical and Experimental Immunology, Supplement
JF - Clinical and Experimental Immunology, Supplement
SN - 0964-2536
IS - 1
ER -
ID: 8114191