The acylation and phosphorylation pattern of lipid A from Xanthomonas Campestris strongly influence its ability to trigger the innate immune response in arabidopsis
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The acylation and phosphorylation pattern of lipid A from Xanthomonas Campestris strongly influence its ability to trigger the innate immune response in arabidopsis. / Silipo, Alba; Sturiale, Luisa; Garozzo, Domenico; Erbs, Gitte; Jensen, Tina Tandrup; Lanzetta, Rosa; Dow, J. Maxwell; Parrilli, Michelangelo; Newman, Mari-Anne; Molinaro, Antonio.
I: ChemBioChem, Bind 9, Nr. 6, 2008, s. 896-904.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - The acylation and phosphorylation pattern of lipid A from Xanthomonas Campestris strongly influence its ability to trigger the innate immune response in arabidopsis
AU - Silipo, Alba
AU - Sturiale, Luisa
AU - Garozzo, Domenico
AU - Erbs, Gitte
AU - Jensen, Tina Tandrup
AU - Lanzetta, Rosa
AU - Dow, J. Maxwell
AU - Parrilli, Michelangelo
AU - Newman, Mari-Anne
AU - Molinaro, Antonio
PY - 2008
Y1 - 2008
N2 - Lipopolysaccharides (LPSs) are major components of the cell surface of Gram-negative bacteria. LPSs comprise a hydrophilic heteropolysaccharide (formed by the core oligosaccharide and the O-specific polysaccharide) that is covalently linked to the glycolipid moiety lipid A, which anchors these macromolecules to the external membrane. LPSs are one of a group of molecules called pathogen-associated molecular patterns (PAMPs) that are indispensable for bacterial growth and viability, and act to trigger innate defense responses in eukaryotes. We have previously shown that LPS from the plant pathogen Xanthomonas campestris pv. campestris (Xcc) can elicit defense responses in the model plant Arabidopsis thaliana. Here we have extended these studies by analysis of the structure and biological activity of LPS from a nonpathogenic Xcc mutant, strain 8530. We show that this Xcc strain is defective in core completion and introduces significant modification in the lipid A region, which involves the degree of acylation and nonstoichiometric substitution of the phosphate groups with phosphoethanolamine. Lipid A that was isolated from Xcc strain 8530 did not have the ability to induce the defense-related gene PR1 in Arabidopsis, or to prevent the hypersensitive response (HR) that is caused by avirulent bacteria as the lipid A from the wild-type could. This suggests that Xcc has the capacity to modify the structure of the lipid A to reduce its activity as a PAMP. We speculate that such effects might occur in wild-type bacteria that are exposed to stresses such as those that might be encountered during plant colonization and disease.
AB - Lipopolysaccharides (LPSs) are major components of the cell surface of Gram-negative bacteria. LPSs comprise a hydrophilic heteropolysaccharide (formed by the core oligosaccharide and the O-specific polysaccharide) that is covalently linked to the glycolipid moiety lipid A, which anchors these macromolecules to the external membrane. LPSs are one of a group of molecules called pathogen-associated molecular patterns (PAMPs) that are indispensable for bacterial growth and viability, and act to trigger innate defense responses in eukaryotes. We have previously shown that LPS from the plant pathogen Xanthomonas campestris pv. campestris (Xcc) can elicit defense responses in the model plant Arabidopsis thaliana. Here we have extended these studies by analysis of the structure and biological activity of LPS from a nonpathogenic Xcc mutant, strain 8530. We show that this Xcc strain is defective in core completion and introduces significant modification in the lipid A region, which involves the degree of acylation and nonstoichiometric substitution of the phosphate groups with phosphoethanolamine. Lipid A that was isolated from Xcc strain 8530 did not have the ability to induce the defense-related gene PR1 in Arabidopsis, or to prevent the hypersensitive response (HR) that is caused by avirulent bacteria as the lipid A from the wild-type could. This suggests that Xcc has the capacity to modify the structure of the lipid A to reduce its activity as a PAMP. We speculate that such effects might occur in wild-type bacteria that are exposed to stresses such as those that might be encountered during plant colonization and disease.
KW - Former LIFE faculty
KW - lipopolysaccharides
KW - mass spectrometry
KW - NMR spectroscopy
KW - structure-activity relationships
KW - Xanthomonas campestris 8530
U2 - 10.1002/cbic.200700693
DO - 10.1002/cbic.200700693
M3 - Journal article
VL - 9
SP - 896
EP - 904
JO - ChemBioChem
JF - ChemBioChem
SN - 1439-4227
IS - 6
ER -
ID: 8104207