The effect of tyramine infusion and exercise on blood flow, coagulation and clot microstructure in healthy individuals
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Standard
The effect of tyramine infusion and exercise on blood flow, coagulation and clot microstructure in healthy individuals. / Lawrence, Matthew J; Davies, Gareth; Nyberg, Michael Permin; Whitley, Janet; Evans, Vanessa; Williams, Rhodri; Hellsten, Ylva; Evans, Phillip A.
I: Thrombosis Research, Bind 170, 10.2018, s. 32-37.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - The effect of tyramine infusion and exercise on blood flow, coagulation and clot microstructure in healthy individuals
AU - Lawrence, Matthew J
AU - Davies, Gareth
AU - Nyberg, Michael Permin
AU - Whitley, Janet
AU - Evans, Vanessa
AU - Williams, Rhodri
AU - Hellsten, Ylva
AU - Evans, Phillip A
N1 - CURIS 2018 NEXS 278
PY - 2018/10
Y1 - 2018/10
N2 - Background: The long term benefits of exercise on the cardiovascular status of a patient have been proven, however, their benefit/risk relationship with exercise intensity is unclear. Furthermore, many thromboembolic diseases such as myocardial infarction and ischaemic stroke are associated with profound catecholamine release. In this study we explore the relationship between catecholamine release and hemodynamic changes and their effect on coagulation.Materials and methods: Twelve healthy recreationally active males were recruited. Local anesthesia was given and catheters were placed under aseptic conditions, in the femoral artery and vein of the experimental leg. The first experiment involved tyramine infusion into the femoral artery at a dose of 1.0 μmol·min-1·L leg volume-1. The second experiment involved single leg knee-extensor exercise performed at 30 W for 15 min. Venous blood was collected at each time point to assess clot microstructure using the df biomarker.Results and conclusions: Tyramine infusion causes a local noradrenaline release in the leg. The increase in noradrenaline was associated with a significant increase in clot microstructure formation (df increased from 1.692 ± 0.029 to 1.722 ± 0.047, p = 0.016). Additionally moderate intensity single leg knee extensor exercise, which minimally alters sympathetic activity, also induced an increases in df (from 1.688 ± 0.025 to 1.723 ± 0.023, p = 0.001). This suggests that exercise can alter clot microstructure formation both via an increase in catecholeamine levels and by factors related to muscle activity per se, such as increased blood flow and consequent shear. These findings have implications for recommendations of exercise in patients at risk of cardiovascular events.
AB - Background: The long term benefits of exercise on the cardiovascular status of a patient have been proven, however, their benefit/risk relationship with exercise intensity is unclear. Furthermore, many thromboembolic diseases such as myocardial infarction and ischaemic stroke are associated with profound catecholamine release. In this study we explore the relationship between catecholamine release and hemodynamic changes and their effect on coagulation.Materials and methods: Twelve healthy recreationally active males were recruited. Local anesthesia was given and catheters were placed under aseptic conditions, in the femoral artery and vein of the experimental leg. The first experiment involved tyramine infusion into the femoral artery at a dose of 1.0 μmol·min-1·L leg volume-1. The second experiment involved single leg knee-extensor exercise performed at 30 W for 15 min. Venous blood was collected at each time point to assess clot microstructure using the df biomarker.Results and conclusions: Tyramine infusion causes a local noradrenaline release in the leg. The increase in noradrenaline was associated with a significant increase in clot microstructure formation (df increased from 1.692 ± 0.029 to 1.722 ± 0.047, p = 0.016). Additionally moderate intensity single leg knee extensor exercise, which minimally alters sympathetic activity, also induced an increases in df (from 1.688 ± 0.025 to 1.723 ± 0.023, p = 0.001). This suggests that exercise can alter clot microstructure formation both via an increase in catecholeamine levels and by factors related to muscle activity per se, such as increased blood flow and consequent shear. These findings have implications for recommendations of exercise in patients at risk of cardiovascular events.
KW - Faculty of Science
KW - Catecholamine
KW - Clot microstructure
KW - Coagulation
KW - Exercise
U2 - 10.1016/j.thromres.2018.07.025
DO - 10.1016/j.thromres.2018.07.025
M3 - Journal article
C2 - 30098458
VL - 170
SP - 32
EP - 37
JO - Thrombosis Research
JF - Thrombosis Research
SN - 0049-3848
ER -
ID: 201043007