Training state and skeletal muscle autophagy in response to 36 h of fasting
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Training state and skeletal muscle autophagy in response to 36 h of fasting. / Dethlefsen, Maja Munk; Bertholdt, Lærke; Gudiksen, Anders; Stankiewicz, Tomasz; Bangsbo, Jens; van Hall, Gerrit; Plomgaard, Peter; Pilegaard, Henriette.
I: Journal of Applied Physiology, Bind 125, Nr. 5, 11.2018, s. 1609-1619.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Training state and skeletal muscle autophagy in response to 36 h of fasting
AU - Dethlefsen, Maja Munk
AU - Bertholdt, Lærke
AU - Gudiksen, Anders
AU - Stankiewicz, Tomasz
AU - Bangsbo, Jens
AU - van Hall, Gerrit
AU - Plomgaard, Peter
AU - Pilegaard, Henriette
N1 - CURIS 2018 NEXS 406
PY - 2018/11
Y1 - 2018/11
N2 - The present study aimed at investigating fasting-induced responses in regulators and markers of autophagy in vastus lateralis muscle from untrained and trained human subjects. Untrained and trained subjects (based on maximum oxygen uptake, muscle citrate synthase activity, and oxidative phosphorylation protein level) fasted for 36 h with vastus lateralis muscle biopsies obtained at 2, 12, 24, and 36 h after a standardized meal. Fasting reduced (P 0.05) skeletal muscle microtubule-associated protein-1A/1B light chain 3 (LC3)I, LC3II, and adaptor protein sequestosome 1/p62 protein content in untrained subjects only. Moreover, skeletal muscle RAC-alpha serine/threonine-protein kinase (AKT) Thr308, AMP-activated protein kinase (AMPK) Thr172, and Unc-51-like autophagy-activating kinase-1 (ULK1) Ser555 phosphorylation state, as well as Bcl-2-interacting coiled-coil protein-1 (Beclin1) and ULK1 Ser757 phosphorylation, was lower (P 0.05) in trained than untrained subjects during fasting. In addition, the plasma concentrations of several amino acids were higher (P 0.05) in trained than untrained subjects, and the plasma concentration profile of several amino acids was different in untrained and trained subjects during fasting. Taken together, these findings suggest that 36-h fasting has effects on some mediators of autophagy in untrained human skeletal muscle and that training state influences the effect of fasting on autophagy signaling and on mediators of autophagy in skeletal muscle. NEW & NOTEWORTHY This study showed that skeletal muscle autophagy was only modestly affected in humans by 36 h of fasting. Hence, 36-h fasting has effects on some mediators of autophagy in untrained human skeletal muscle, and training state influences the effect of fasting on autophagy signaling and on mediators of autophagy in skeletal muscle.
AB - The present study aimed at investigating fasting-induced responses in regulators and markers of autophagy in vastus lateralis muscle from untrained and trained human subjects. Untrained and trained subjects (based on maximum oxygen uptake, muscle citrate synthase activity, and oxidative phosphorylation protein level) fasted for 36 h with vastus lateralis muscle biopsies obtained at 2, 12, 24, and 36 h after a standardized meal. Fasting reduced (P 0.05) skeletal muscle microtubule-associated protein-1A/1B light chain 3 (LC3)I, LC3II, and adaptor protein sequestosome 1/p62 protein content in untrained subjects only. Moreover, skeletal muscle RAC-alpha serine/threonine-protein kinase (AKT) Thr308, AMP-activated protein kinase (AMPK) Thr172, and Unc-51-like autophagy-activating kinase-1 (ULK1) Ser555 phosphorylation state, as well as Bcl-2-interacting coiled-coil protein-1 (Beclin1) and ULK1 Ser757 phosphorylation, was lower (P 0.05) in trained than untrained subjects during fasting. In addition, the plasma concentrations of several amino acids were higher (P 0.05) in trained than untrained subjects, and the plasma concentration profile of several amino acids was different in untrained and trained subjects during fasting. Taken together, these findings suggest that 36-h fasting has effects on some mediators of autophagy in untrained human skeletal muscle and that training state influences the effect of fasting on autophagy signaling and on mediators of autophagy in skeletal muscle. NEW & NOTEWORTHY This study showed that skeletal muscle autophagy was only modestly affected in humans by 36 h of fasting. Hence, 36-h fasting has effects on some mediators of autophagy in untrained human skeletal muscle, and training state influences the effect of fasting on autophagy signaling and on mediators of autophagy in skeletal muscle.
KW - Faculty of Science
KW - Fasting
KW - Exercise training
KW - Skeletal muscle
KW - Autophagy
KW - Signaling
KW - Plasma amino acid
KW - Autophagy
KW - Exercise training
KW - Fasting
KW - Plasma amino acid
KW - Signaling
KW - Skeletal muscle
KW - autophagy
KW - signaling
KW - plasma amino acid
KW - exercise training
KW - fasting
KW - skeletal muscle
U2 - 10.1152/japplphysiol.01146.2017
DO - 10.1152/japplphysiol.01146.2017
M3 - Journal article
C2 - 30161009
VL - 125
SP - 1609
EP - 1619
JO - Journal of Applied Physiology
JF - Journal of Applied Physiology
SN - 8750-7587
IS - 5
ER -
ID: 201903004