Breastmilk lipids and oligosaccharides influence branched short-chain fatty acid concentrations in infants with excessive weight gain
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Breastmilk lipids and oligosaccharides influence branched short-chain fatty acid concentrations in infants with excessive weight gain. / Pekmez, Ceyda Tugba; Larsson, Melanie Wange; Lind, Mads Vendelbo; Manjarrez, Natalia Vazquez; Yonemitsu, Chloe; Larnkjær, Anni; Bode, Lars; Mølgaard, Christian; Michaelsen, Kim F.; Dragsted, Lars Ove.
In: Molecular Nutrition & Food Research, Vol. 64, No. 3, 1900977, 2020.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Breastmilk lipids and oligosaccharides influence branched short-chain fatty acid concentrations in infants with excessive weight gain
AU - Pekmez, Ceyda Tugba
AU - Larsson, Melanie Wange
AU - Lind, Mads Vendelbo
AU - Manjarrez, Natalia Vazquez
AU - Yonemitsu, Chloe
AU - Larnkjær, Anni
AU - Bode, Lars
AU - Mølgaard, Christian
AU - Michaelsen, Kim F.
AU - Dragsted, Lars Ove
N1 - This article is protected by copyright. All rights reserved.
PY - 2020
Y1 - 2020
N2 - Scope: We aimed to identify breastmilk components associated with fecal concentration of SCFAs and to investigate whether they differ between infants with high weight gain (HW) and normal weight gain (NW).Methods and results: Breastmilk and fecal samples were collected from mother-infant dyads with HW (n = 11) and NW (n = 15) at 5 and 9 months of age. Breastmilk was profiled using an untargeted method on UPLC-qTOF-MS. Fecal SCFAs were quantified using an isotope-labeled chemical derivatization method. Human milk oligosaccharides (HMOs) were quantified using HPLC after fluorescent derivatization. We found lower levels of α-linolenic acid, oleic acid, 3-oxohexadecanoic acid, LPE (P-16:0), LPC (16:0), LPC (18:0), PC (36:2) in breastmilk from mothers from the HW-group at 5 months of age. Fecal SCFA concentrations were increased during the transition period from breastfeeding to complementary feeding. Fecal butyrate concentration was higher in the NW-group at 9 months of age. Fecal branched SCFAs were positively associated with breastmilk phospholipid levels, free-fatty acid levels, HMO-diversity, sialylated-HMOs, 6'-sialyllactose, and disialyl-lacto-N-hexaose.Conclusion: Fecal branched SCFA concentrations seem to be affected by breastmilk lipid and HMO composition. These differences in breastmilk phospholipids, α-linolenic acid, and specific HMO structures may partially explain the excessive weight gain in early life.
AB - Scope: We aimed to identify breastmilk components associated with fecal concentration of SCFAs and to investigate whether they differ between infants with high weight gain (HW) and normal weight gain (NW).Methods and results: Breastmilk and fecal samples were collected from mother-infant dyads with HW (n = 11) and NW (n = 15) at 5 and 9 months of age. Breastmilk was profiled using an untargeted method on UPLC-qTOF-MS. Fecal SCFAs were quantified using an isotope-labeled chemical derivatization method. Human milk oligosaccharides (HMOs) were quantified using HPLC after fluorescent derivatization. We found lower levels of α-linolenic acid, oleic acid, 3-oxohexadecanoic acid, LPE (P-16:0), LPC (16:0), LPC (18:0), PC (36:2) in breastmilk from mothers from the HW-group at 5 months of age. Fecal SCFA concentrations were increased during the transition period from breastfeeding to complementary feeding. Fecal butyrate concentration was higher in the NW-group at 9 months of age. Fecal branched SCFAs were positively associated with breastmilk phospholipid levels, free-fatty acid levels, HMO-diversity, sialylated-HMOs, 6'-sialyllactose, and disialyl-lacto-N-hexaose.Conclusion: Fecal branched SCFA concentrations seem to be affected by breastmilk lipid and HMO composition. These differences in breastmilk phospholipids, α-linolenic acid, and specific HMO structures may partially explain the excessive weight gain in early life.
KW - Faculty of Science
KW - Isobutyrate
KW - Isovalerate
KW - 2-methylbutyrate
KW - Gut fermentation
KW - Proteolytic bacteria
U2 - 10.1002/mnfr.201900977
DO - 10.1002/mnfr.201900977
M3 - Journal article
C2 - 31801176
VL - 64
JO - Molecular Nutrition & Food Research
JF - Molecular Nutrition & Food Research
SN - 1613-4125
IS - 3
M1 - 1900977
ER -
ID: 231553047